Preparing the Instrument to Run Samples
26
P/N CLS151164 Rev. B
LabChip GX Touch/GXII Touch User Manual
PerkinElmer, Inc.
Calibrating the Instrument to Run Protein Clear
HR Assays
If a Protein Clear HR chip is inserted into the instrument, the user
must calibrate the instrument to run a Protein Clear HR assay
before starting the run. Calibration is performed by processing a
known protein sample, and uses the results to precisely set the
electrode currents for that assay to obtain consistent chip-to-chip
assay results.
To calibrate the instrument to run Protein Clear HR assays:
1
Insert the Protein Clear HR chip (see
) OR
Open the LabChip GX Touch software (see
) with the
chip already inserted into the instrument.
2
Touch the
Prime and Calibrate
button on the
.
The
opens.
3
Select the plate type from the
Select Plate Type
drop-down list.
4
Select the assay from the
Select Assay
drop-down list. (To
select the location of the assay file, touch the
Change Assay
Folder
button and select the assay folder on the
.)
5
Touch the
Select Well Containing Standard Sample
button to
select the well that contains the VeriMAb
sample.
6
Select the desired well from the full-size plate diagram and
touch the
Done
button.
7
Touch the
Prime and Calibrate
button on the
.
•
If the calibration is successful, a dialog box opens indicating
that the instrument is now calibrated for the Protein Clear
HR assay. Touch the
OK
button.
•
If the calibration fails, a dialog box opens indicating that the
calibration process failed and a Protein Clear HR assay run
cannot be performed. Touch the
OK
button. Perform the
recommended actions on
and retry the calibration.
•
If the calibration is outside the % Purity target range or if
dips from the Egrams could not be eliminated, a dialog box
opens indicating that the calibration is marginal. Touch the
Yes
button to run the chip despite the issue displayed on the
dialog box. Touch
No
to fix the issue. Perform the
recommended actions on
and retry the calibration.
8
To retry the calibration, select the
Skip Prime Step
check box
on the
. The chip is calibrated but
not primed.
