Cypher SPM
User Guide
3. Start imaging under the conditions (speed, scan size, and so on) you eventually plan to use on
the sample which was mounted and sealed inside the glovebox.
4. Observe image quality while you experiment with the inert gas flow and note the maximum
flow before imaging noise becomes noticeable.
5. Turn off the gas flow.
6. Stop imaging and disengage the tip.
7. Remove the cantilever holder.
8. Remove the entire sample cell from the AFM.
See
Exchanging the sample stage and cell body on page 84
.
12.2 Preparing the cell and gas lines
We will completely bypass the gas valve and pressure manifold for this demonstration.
1. Remove and store the gas lines that were already present on your Cypher AFM.
See
Gas handling and leak testing on page 93
2. Store the tubing sections in a safe place.
3. Consult the Cypher ES accessory parts list (see
Environmental scanner parts list on page 44
) and
find the following:
l
231.006 1/16” O.D. Tubing.
l
231.008 Luer Fittings: 231.008.
l
5cc Syringe: 080.010.
l
Threaded fittings: 232.015.
l
Scalpel knife with blade, 290.147 and 290.148
l
Ferrules: 232.016.
l
Extender Tool Fitting Wrench: 114.800.
l
Fitting Compression Fixture: 114.721
4. Locate a three way valve.
l
The luer-type valve shown is provided by Asylum.
5. Cut two sections of tubing of approximately 0.5 m
They should be long enough to reach from the scanner cell to the outside of the ES enclosure.
12 Glovebox Protocols
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