Cypher SPM
User Guide
Locate cantilever in image:
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The goal of this step is just to get the cantilever into the field of view. Use the four arrows
at the top left of the video window to look for the edge of the cantilever chip and/or the
cantilever. As mentioned in the previous step, most likely the cantilever will not be per-
fectly in focus.
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If you are oriented such that you are sitting directly in front of the Cypher microscope,
hitting the left arrow will move the objective to your left, while hitting the top arrow will
move the objective away from you.
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If you see nothing at all in the field of view, most likely the cantilever chip is located to
the left of the field of view. Hit the left arrow to move the objective towards the left and
look for the cantilever chip edge.
See
for
example
images.
9.
Set the tip focus position:
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Under the Approach tab on the Engage Panel select the Move Focus (on the left, red) large pic-
ture button.
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Use the on screen arrow buttons until cantilever is in focus. Single arrows are slow; double arrows
are fast.
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Click Set next to Focus on Tip button (on the right, blue).
Important
The cantilever is at an 11º angle and the whole lever cannot be in focus at once. Bring the end of
the cantilever closest to the tip in focus.
10.
Optional image enhancement and zoom, particularly useful for small cantilevers:
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If you want to see the image with more resolution, select Decimate 1 from the Options pull-down
menu. This brings all the pixels down from the video camera but will slow the screen update rate.
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To the left of the Options menu is a Zoom button. This button, once clicked, will change the
cursor into a magnifying glass. Click on the cantilever to get an enlarged view.
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Both of these items may improve your ability to focus from the previous step. If you do refocus,
be sure to click Set next to the Focus on Tip button.
11.
Center laser spot on cantilever:
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Click on the Spot On button at the top left of the video window. The mouse pointer will acquire
some small red lines.
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Now click on the center of the cantilever (see figure to right).
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Alternately, right-click on the center of the cantilever and then select the Spot On option.
12.
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24 Engaging the cantilever on the sample
