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Oxford Instruments Cypher SPM, User Manual

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Oxford Instruments Cypher SPM User Manual Download Page 62

Cypher SPM

User Guide

10

10

Cell Body And Sample Stage Guide

The sealed environment around the sample and cantilever is formed by three components:

l

The cantilever holder forms the lid.

l

The cell body forms the sides.

l

The sample stage forms the bottom.

A Perfusion Cantilever Holder, Fluid Cell Body,

and Ambient Sample Stage, disassembled.

A fully sealed assembly.

10.1 Gas cell

Part #

Holder Description

Front Photo

Back Photo

901.746

The gas cell is meant to be
used primarily for gas envir-
onments as it has electrical
feedthroughs. However, it
can also be used in a
droplet environment.

The gas cell has three magnetic contacts which can be used to route electrical signals to the sample.

10 Cell Body and Sample Stage Guide

Page 62 of 243

 
 

 

Summary of Contents for Cypher SPM

Page 1: ...Cypher SPM User Guide Part No XX XXXX XXX...

Page 2: ...vided without warranty of any kind either implied or expressed including but not limited to the implied warranties of merchantability and fitness for a particular purpose Copyright Information contain...

Page 3: ...ser safety interlocks 14 2 6 Power supply safety and thermal management 16 2 6 1 Power supply and earthing 16 2 6 2 Overheating 17 2 7 Disposal 17 2 8 Cleaning 17 3 Contact Shipping and Support Inform...

Page 4: ...nvironmental scanner cantilever holders 54 9 1 Visual identification of ES cantilever holders 54 9 1 1 ORCA cantilever holder kit parts list 55 9 2 Electronic identification of ES cantilever holders 5...

Page 5: ...2 Prepare the scanner 86 10 9 3 Remove the sample stage and chamber 86 10 9 4 Mounting the new stage and cell combination 88 10 9 5 Replacing the scanner 90 10 10 Storing sample stages and cell bodie...

Page 6: ...ilter cube 132 16 7 blueDrive software interface 134 16 7 1 Turning on blueDrive 134 16 7 2 39 5 3 Positioning the blue spot 135 16 7 3 39 5 4 Acquiring a cantilever tune 137 16 7 4 Operating on the t...

Page 7: ...d scanner 198 30 2 Loading the cantilever and sample on an environmental scanner 205 30 3 AC mode imaging in air with the standard scanner 214 30 4 AC mode imaging in air with the environmental scanne...

Page 8: ...Part I Getting Started System safety and overview...

Page 9: ...Confidential information Information contained in this manual is proprietary and remains the intellectual property of Oxford Instruments NanoAnalysis The manual is distributed by Oxford Instruments N...

Page 10: ...ionizing radiation exists 2 2 General safety precautions To avoid fire hazard and risk of electric shock l Do not use the equipment if any cable is damaged l Do not operate with suspected damage or f...

Page 11: ...ctive lens along with its carriage Always keep your hands clear of the Cypher SPM when performing any software or manually controlled motor moves 2 4 1 Avoiding unsafe situations The following image i...

Page 12: ...turns are required for every millimeter of objective motion Turn clock wise to move the objective down and counter clockwise to move the objective up 2 5 Light source safety Use of controls or adjust...

Page 13: ...he only place a person can be exposed to the light is where the light exits the object ive There are numerous interlocks to minimize the possibility of exposure However you should avoid the following...

Page 14: ...and off by a control voltage which originates inside the ARC2 controller On its way to the laser the voltage passes through various safety switches as shown in the fol lowing image The relevant parts...

Page 15: ...he user To prevent this the laser power circuit is broken as soon as the scanner cable is disconnected a necessary step for scanner removal Laser module dual magnetic switch interlock 4 5 The laser mo...

Page 16: ...s been unplugged for at least 10 minutes Adhere to the fuse ratings appropriate to the main supply voltage listed on the back side of the ARC2 controller Not following the recommended ratings may caus...

Page 17: ...ooks on top of the backpack 2 7 Disposal European Directive on Waste Electrical and Electronic Equipment WEEE When disposing of the system or any of its components users in the European Union should c...

Page 18: ...Subject to the availability of components Oxford Instruments NanoAnalysis shall use reasonable endeavours to provide support for the Cypher SPM for a period of seven years after the date of last prod...

Page 19: ...o the ARC2 and a USB 2 0 directly to the Cypher SPM For further information see Recommended USB ports on page 23 The ARC2 Asylum Research Controller 2 is referred to as the controller It houses power...

Page 20: ...ature can be controlled Keeping the microscope at a constant temperature is important for maintaining long term control of the relative position between the cantilever and the sample 4 1 2 Chassis The...

Page 21: ...ble module that provides a top down optical view of the cantilever and sample It is comprised of a tube lens Koehler illumination with an LED source and a digital camera The camera module uses the obj...

Page 22: ...and mm Item Part Item Description Qty Picture 1 080 122 15 mm AFM Specimen Disc Also avail able from Ted Pella part number 16218 50 2 290 101 2A Tweezer SA Tapered Round Blunt Standard Grade 1 3 290...

Page 23: ...6 and also fits the cantilever holder changing stations 5 4 3 Recommended USB ports Under some circumstances using a USB memory stick or portable USB drive results in one or more communication errors...

Page 24: ...ARC into the bottom right USB ports as shown in the following image Note Do not plug the Cypher or ARC into the USB 3 0 ports marked SS for SuperSpeed Plug the mouse and keyboard into the top USB por...

Page 25: ...eft USB port Plug the mouse into the bottom left USB port USB 2 0 and 3 0 devices like USB drives and printers and LCD monitor hubs may now safely be plugged into the remaining non circled ports inclu...

Page 26: ...e commercially available software package Igor Pro which is developed by WaveMetrics Igor Pro itself has nothing to do with scanning probe microscopes Rather it is a stand alone program that has exten...

Page 27: ...monitor of your system Refer to the screen shot in 1 3 as we introduce the various controls and data displays We ll go clockwise from the upper left Note that if you are viewing this file on a comput...

Page 28: ...rols the entire process of the cantilever approach to the sample Its three tabs control Approach Motorized approach of cantilever and microscope objective toward the sample Detector Centering of refle...

Page 29: ...plete list of all offline controls Status Bar Along the bottom of the screen Icon controls relate to the status of connected instrument com ponents The low level software version is also displayed We...

Page 30: ...ould be illuminated and the software initializes Make sure your fingers are clear from any pinch points before homing the motors see Avoiding unsafe situations on page 11 You can abort the homing proc...

Page 31: ...Cypher SPM User Guide 3 Turn off the laser key on the controller 4 Power off the controller Page 31 of 243 5 Starting and stopping the system...

Page 32: ...Part II Standard Scan ner This part of the user guide is the principal reference for operating the Cypher SPM instru ment with a standard scanner...

Page 33: ...ctuator and sensor for the sample Z motion l A cantilever holder and mechanical means for engaging the cantilever with the sample surface Since Cypher s scanners are whole AFMs unto themselves each co...

Page 34: ...antilever is superior to other cypher scanner models Many standard scanner cantilever holders allow for a variety of imaging modes The Scanner itself comes in regular and high voltage models The follo...

Page 35: ...oltage to the cantilever Many more contain specific electronics allowing for current measurement application of high voltage to the tip and more Note Cantilever holders are the most delicate component...

Page 36: ...air and droplets Fits in the Droplet Changing Station For use in air or liquid 901 727 STM Scanning Tunneling Micro scopy Fits in the Air Changing Station For use in air or liquid 901 73x ORCA Conduc...

Page 37: ...s list on page 55 and The ORCA Amplifier on page 40 Its use is described in Con ductive AFM ORCA 901 714 High Voltage Typically used for High Voltage PFM Imaging Fits in the Air Changing Station For u...

Page 38: ...ever and sample on a standard scanner on page 198 2 In the AZtec AFM software select Programming Cantilever Holder and Sample Panel XXX 3 Click Check Holder and the type of cantilever holder is highli...

Page 39: ...rements described in this section 1 3 823 009 HOPG sample Used as a conductive AFM test sample 1 4 448 079 Sample bias wire assembly Connects sample to voltage source on top of the scanner 6 5 208 05...

Page 40: ...10 V so multiplying the sensitivity by 10 will give you the full range The ORCA amplifier incorporates the use of a trans impedance amplifier which converts the input current from the tip to an output...

Page 41: ...hannel panel like the single gain ORCA holder The output of the current to voltage amplifier s first stage has a lower gain more total current range signal is monitored as Current 2 from the master ch...

Page 42: ...Part III Environmental Scan ner This part of the user guide is the principal reference for operating the Cypher SPM instru ment with an environmental scanner...

Page 43: ...ple l An actuator and sensor for the sample Z motion l A cantilever holder and mechanical means for engaging the cantilever with the sample surface Since Cypher s scanners are whole AFMs unto themselv...

Page 44: ...photos is in cm and mm Item Part Item Description Qty Picture 1 290 147 Scalpel Handle Used to attach liquid perfusion tubing to the cantilever holder Also useful when trimming thicker tubing for gas...

Page 45: ...ed to perfuse fluid 4 7 080 010 5 cc syringe HSW Typically used for fluid or gas perfusion 4 8 231 006 PFA Tubing 1 16 OD 0 040 ID 5 ft package Special large bore tubing for gas perfusion 2 9 231 028...

Page 46: ...t Used with 1 16 fittings IDEX part number M660 6 14 114 721 Fitting Compression Fixture Required to attach fitting ferrules to 1 16 tubing 1 15 290 165 Platypus Tweezers EMS part 78317 style 2AZ Usef...

Page 47: ...S 00 90 x 1 8 screw Spare screws used to fasten the sample stage membrane to the cell body 12 21 230 040 FKM O ring 1 5 mm x 0 5 mm 75 A Durometer Now obsolete use 230 039 instead 0 22 230 039 FKM O r...

Page 48: ...er 1 28 xxx xxx 1 8 OD X 1 16 ID vinyl tubing Used to connect to the gas flow rotameter 6 ft 29 114 801 Thermal pad spare Adheres to the back of the environmental scanner Use as a replacement in case...

Page 49: ...448 140 Electrical Sample Puck Assembly Used to electrically bias or ground a sample Includes Puck Bias Lead wire 448 139 and Modified 000 120 screw 114 853 3 36 901 778 ES tubing kit Pre made tubing...

Page 50: ...cause serious damage to your can tilever holder and sample stage 2 Never load non flat samples into the ES scanner l Unlike the S scanner the ES scanner cannot accept non flat samples A non flat samp...

Page 51: ...gh it is not required lowering the cantilever holder stage fully with the cantilever holder removed will make it easier to exchange samples without touching the heater stage Note Pay attention If you...

Page 52: ...ge Fully depress the spring until you feel the hard stop l After fully engaging the spring loaded stop at the back of the scanner lock the scanner in place Note Proper seating of the scanner is most i...

Page 53: ...User Guide When you no longer require these O rings please store them back in the case FFKM Costs between 10 and 100 times more than FKM so do not misplace them Page 53 of 243 8 Environmental Scanner...

Page 54: ...Nearly all have the ability to apply a voltage to the cantilever Many more contain specific electronics allowing for current measurement application of high voltage to the tip and more Note Cantilever...

Page 55: ...parts list below and The ORCA Amplifier on page 40 901 771 High Voltage Typically used for High Voltage PFM Imaging 901 777 STM Scanning Tunneling Micro scopy 901 763 AMFM 9 1 1 ORCA cantilever holder...

Page 56: ...to voltage source on top of the scanner 6 5 208 05 Samarium Cobalt Magnet 0 07 D X 0 104 L Used to connect the bias wire to the sample 6 6 290 160 Leitsilber Conductive Paint 0 5 Oz Used to conductive...

Page 57: ...der is highlighted 9 3 Changing the cantilever clip Only the cantilever clip can be removed from the cantilever holder Other parts must not be removed or serviced by the user 9 3 1 Removing the ES can...

Page 58: ...ever holder should be sitting ready without a clip as shown 2 Prepare the clip l Put the screw clip and O ring together as shown l Screw only a few turns into the threaded hole in the glass of the can...

Page 59: ...This will be the O ring compressing l If the clip still has a tendency to turn around during tightening of the screw consider hold ing the clip pressed down right photo so it seats properly in the cut...

Page 60: ...fluid ports before proceeding to the next step 3 Add cleaning fluid l Pour a small amount of solvent or water into the cup We recommend ethyl or isopropyl alcohol or deionized water Note Acetone and M...

Page 61: ...Cypher SPM User Guide 9 5 Storage After cleaning store the cantilever holder in the membrane box in which it originally shipped Page 61 of 243 9 Environmental scanner cantilever holders...

Page 62: ...ntilever Holder Fluid Cell Body and Ambient Sample Stage disassembled A fully sealed assembly 10 1 Gas cell Part Holder Description Front Photo Back Photo 901 746 The gas cell is meant to be used prim...

Page 63: ...n cm and mm 1 901 746 Gas Cell Body 1 2 114 916 Boot Clamp Ring 8 Bolt Pattern Con nects to the bottom of the cell to form the seal between the cell body and the sample stage membrane 1 3 00 90 x 1 8...

Page 64: ...Borosilicate Glass Contacts Nicked or Gold Plated Other Epoxy Cleaning The whole cell body can be immersed or sonicated in ethyl or isopropyl alcohol or water Do not use acetone or methylene chloride...

Page 65: ...tead of raising it When you lower the cantilever holder you can crash the can tilever holder into the sample and cause serious damage to the scanner 2 Unlock the scanner and pull it all the way forwar...

Page 66: ...weezers grip the con nector as shown 6 Insert the connector l Carefully insert the connector as shown 7 Insert the smaller connector l Insert the smaller connector as shown on the left l The final cab...

Page 67: ...holders it is not possible to engage on the sample shown on the left since the magnet would hit the cantilever holder before the cantilever engaged on the sample l For electrical measurements use the...

Page 68: ...rd gas cell cable 448 137 There are other cables which assign the magnet connections differently l If you do not have a connector then use the cross point switch panel to route one of the BNC inputs f...

Page 69: ...cell body 8 4 1 72 x 0 25 SHCS SS 1 72 x 1 4 long screw Used to lock down the cantilever holder 2 10 2 2 Fluid cell specifications Exposed materials Cell wall Borosilicate Glass Cleaning The whole ce...

Page 70: ...e cell s interior The two elec trodes located on either side of the sensor opening provide electrical connections to analog ground and sample bias Note The stock applications board is configured this...

Page 71: ...4 8 4 1 72 x 0 25 SHCS SS 1 72 x 1 4 long screw Used to lock down the cantilever holder 2 5 458 257 1 Applications Board Used to connect the Humidity sensor to the scanner electronics 1 10 3 2 Humidit...

Page 72: ...s 6 Secure the stage base into the scanner Note The humidity cell is delivered with an Ambient Stage already installed The humidity cell body is compatible with all the Environmental Scanner Stages If...

Page 73: ...nd the cell body l Verify that the devices are now present in the device list l Note You may need to update the version of the software to version 14 16 136 or higher 9 Record the humidity l The humid...

Page 74: ...ottom View 10 4 1 Ambient sample stage parts list There are no associated parts The stage comes by itself and has part number 901 761 10 4 2 Ambient sample stage specifications Exposed materials Membr...

Page 75: ...stage is the first choice for imaging at room temperature Heater Stage 10 5 1 Heater sample stage parts list There are no associated parts The stage comes by itself and has part number 901 747 10 5 2...

Page 76: ...Holder Description Front Photo Back Photo 901 748 For imaging at 0 C to 120 C Safe for gas and fluid operation The ambient sample stage is the first choice for imaging at room temperature Top Surface...

Page 77: ...ers for cell exchange and handling In this section we provide a list of pitfalls to avoid during use of the Cypher ES for imaging It is intended for experienced Cypher S users who have had only basic...

Page 78: ...ign the cantilever clip with the pocket before tightening the clip will break the cantilever holder glass 4 Use extra care when loading the Heater sample stage l The heater sample stage is extremely f...

Page 79: ...ns l Turning the locking screw more than three turns may cause the screw to come all the way out l If the screw comes all the way out you may need to lean the scanner forward until the screw slides ou...

Page 80: ...g the sample stage and chamber 1 Locate the 1 7 mm slotted screwdriver and a pair of curved tweezers 2 Remove the two cantilever locking screws as shown and lay them aside 3 Place the assembly as show...

Page 81: ...y puncture the membrane with the screwdriver 5 Remove the retaining ring and set it and the screws aside 6 Remove the sample stage l Lift off the sample stage l Your parts should now be as shown to th...

Page 82: ...n those with the three pins on the bottom of the cell The parts can only go together one way l Assist the diaphragm so the pins go through the holes 3 Place the ring l As shown in the photos place the...

Page 83: ...around once more and tighten firmly while holding the tool only with fingertips to prevent over tightening Note Be careful not to slip and possibly puncture the membrane with the screwdriver 6 Your pa...

Page 84: ...approximately three hours This procedure is designed to be performed not merely read If possible perform the procedure under the supervision of an experienced user The Cypher is a research grade instr...

Page 85: ...and gas cell body combination on the right 10 9 1 Required materials You need the following items l Tweezers preferably with curved tip for example Asylum Part 290 102 l 1 16 Hex Driver Asylum Part 2...

Page 86: ...Removing the scanner on page 109 10 9 3 Remove the sample stage and chamber 1 Loosen the sample stage clamping screw l Insert the 5 64 hex driver into the screw hole at the front of the scanner l Turn...

Page 87: ...039 which may or may not come out when you pull out the gas lines 5 Check the gas lines to see if the small O rings are stuck to the end Usually the rings stay inside the cell but in case they are st...

Page 88: ...e O rings if necessary l If no O ring 230 039 is present place it in the bot tom of the port up against the glass l If the O ring is not properly centered remove it preferably with a sharp wooden stic...

Page 89: ...the dovetail connection at the bottom of the stage into the receiving hole on top of the scanner l Press lightly onto the top of the sample stage until the sample stage sinks down into the hole l Chec...

Page 90: ...stage through the windows on the sides of the cell and make sure you cannot see it If the sample stage is not seated properly you will crush it when you go to insert the cantilever holder 10 9 5 Repl...

Page 91: ...10 10 1 Storing sample stage and cell body combinations Since cell bodies must always be used while attached to a sample stage we recommend storing them together when possible For this purpose the cel...

Page 92: ...s shown with the cutout placed where the cable exits the heater stage 4 Close the box l Close the box and latch it l Inspect the other side to see that the diaphragm is not overly deformed 10 Cell Bod...

Page 93: ...es to the lower left of the scanner One of the lines branches off to a pressure sensor and then passes through a com puter controlled valve Depending on the application this valve may be open or close...

Page 94: ...in the tool slides over the tubing l Unscrew until the fitting comes loose An O ring should stay attached to the tubing If not you may need to retrieve it from the port with tweezers l If it feels the...

Page 95: ...n the tubing has been replaced reverse attach the manifold again with its screws 11 3 Manifold lab side connection 1 Locate the manifold port l The lab side port of the valve manifold is shown in the...

Page 96: ...uld prevent any damage to the fitting 4 Guide the tubing l Press the tubing into the guides Tip The tubing is not perfectly round and it may fit better if it is twisted a bit 11 4 Gas perfusion imagin...

Page 97: ...as soon as the cell is mounted on the AFM Note Imaging electrically insulating samples under conditions of very low water vapor can lead to unwanted forces on the cantilever due to static electric ch...

Page 98: ...is demonstration 1 Remove and store the gas lines that were already present on your Cypher AFM See Gas handling and leak testing on page 93 2 Store the tubing sections in a safe place 3 Consult the Cy...

Page 99: ...own in the diagram 10 Seat the fitting as follows l Attach the red Luer Tight seating tool which has a piece of red plastic embedded into it to the female Luer Tight fitting by screwing it firmly onto...

Page 100: ...a later step l If there is no hole here you can proceed without using the screw but should consider contacting sup port asylumresearch com to see if your holder can be updated 2 Place all the collect...

Page 101: ...usually is dur ing use 7 Attach the bar l Using two longer screws 1 72 x attach the metal bar as shown l Tighten the screws very gently and keep the bar from tilting Tightly securing only one screw m...

Page 102: ...t side and take the cell to the AFM Tip Some customers like to first place their cell and tubing inside of a jar like the dessicator shown on the right This gives you even more time and peace of mind...

Page 103: ...o handle the tool 3 Press down and seat the cell body l Press down on the sample stage as shown You will see the membrane bulge a bit under the building pressure This pressure will assist the proper f...

Page 104: ...ll be used to secure the cantilever holder in the next step During these steps the cantilever holder could be pulled loose exposing your sample 6 Rotate the cantilever holder into position and secure...

Page 105: ...osphere but there is not sufficient time to contaminate the sample chamber l The next step creates gas flow that continuously purges to atmosphere 10 Turn the valve clockwise to divert gas flow throug...

Page 106: ...Part IV Instrument Components This part of the user guide covers the description installation and exchange of hardware components of the instrument scanner optics laser module and blueDrive...

Page 107: ...gnification is fixed Only digital zooming is possible within the software Depending on the imaging conditions air fluid a correction collar on the objective must be adjus ted The procedures in this us...

Page 108: ...era is focused in the same plane as the laser spot used to detect cantilever motion which usu ally brings the back of the cantilever into focus In most cases a focused view of the sample and not the b...

Page 109: ...the environmental scanner The steps are the same for any other model 1 Raise the coarse engage stage l Rotate the Engage Control Knob on the Cypher clock wise and hold it until the cantilever is at it...

Page 110: ...the scanner with this power supply active 4 Unlock the scanner by lifting the lever to the right of the scanner 5 Begin to pull the scanner out l Pull the scanner out about halfway l Clear a place to...

Page 111: ...to its storage loc ation 14 2 Scanner storage The scanner should be stored under basic laboratory conditions preferably in a locked cabinet or drawer where it will not collect dust be accidentally kn...

Page 112: ...canner as shown and set it on the rails l Push it in far enough so it sits stably on the rails 7 Secure the scanner connector l Press the connector into its mating socket l Turn the knob on the right...

Page 113: ...on as shown l The light must remain green 11 If you are prompted to re home the motors during the start up process please do so 12 When the process completes click The popup list of attached hardware...

Page 114: ...aging techniques but some interference effects which can lead to background oscillations when per forming force curve measurements l Super luminescent diodes Also known as SLDs these are the best all...

Page 115: ...er SPM User Guide Part Item Description Picture 901 601 SLD assembly standard spot size 901 602 SLD assembly small spot size 901 603 Laser diode assembly standard spot size Page 115 of 243 15 Light tu...

Page 116: ...e from the scanner 2 Lower the cantilever holder l Rotate the Engage Control Knob counter clockwise to lower the tip to a close distance 1 mm above the top of the scanner This enables easy access to t...

Page 117: ...tube you are removing Note You must store the light tube as soon as it is removed from the SPM 6 Locate the light tube that you are installing 7 Locate the light tube l Look in the area where you just...

Page 118: ...the light tube reversing the removal process into the head making sure that the tube is fully seated Tip The tab on the left side of the tube will key the rotation of the tube in the head 14 Tighten...

Page 119: ...ht tube incorrect for your system 15 3 1 Readjusting the Spot On calibration The Spot On software routine relies on two parameters stored in the light tube calibration inform ation block info block Th...

Page 120: ...Use the arrow markers at the top of the video win dow to steer the spot off the cantilever l Click the Spot On button at the top left of the video window l Click in the center of the cantilever and c...

Page 121: ...cable 3 Replacing the enclosure cover below 15 4 1 Plugging the light tube cable back in MISSING CONTENT XXX 15 4 2 Replacing the enclosure cover The enclosure top is the only thing preventing your Cy...

Page 122: ...st the position of the cover until it fits on the enclosure without any force applied 7 Close right enclosure door l Close the right enclosure door l While pressing it firmly closed to compress the ru...

Page 123: ...Cypher SPM User Guide 11 Replace View System bracket l Replace the bracket in front of the view system l Tighten the screws Page 123 of 243 15 Light tubes...

Page 124: ...hermal excitation In addition to the infrared detection laser 850 nm a blue laser 405 nm is focused on the base of the cantilever causing photothermal excitation local heating of the cantilever leads...

Page 125: ...nar AFM Imaging and Nanomechanics with New blueDrive Photothermal Excitation 16 2 How much light power do you need Depending on the cantilever shape the coating or the imaging environment up to 10 mW...

Page 126: ...it comes premounted onto the Cypher head 1 2 900 266 01 05 Filter cubes 900 266 01 05 labelled by their gain factor 0 01x 0 03x 0 10x 0 30x or 1 00x 5 3 934 005 blueDrive Accessory Kit 290 169 Hex Key...

Page 127: ...the blue wire con nector mounts into the daughter head board from the back 2 Attach blueDrive Optics Cover optional l The blueDrive Optics Cover 901 803 magnetically attaches to the View Module bridg...

Page 128: ...he arrow Do not apply torque to the filter cube 2 Install a new filter cube as follows l Locate the filter cube in its final position l Ensure that the gauge pins line up with the holes on the blueDri...

Page 129: ...nto the indexing holes This may lead to a laser spot positioning error of several tens of microns l To ensure high accuracy positioning of the blue spot between changing filter cubes apply a small for...

Page 130: ...old the screws while unscrewing them and place tissue paper underneath to prevent screws from falling into Cypher hardware as shown on the right 2 Remove laser diode module l Lower the Cypher Head to...

Page 131: ...r installation of a blue laser module the blue laser spot must be focused such that its focal plane matches the focal plane of the infrared laser The following procedure ensures the best focus 1 Enabl...

Page 132: ...how Reset red blue SpotOn Menu Item 16 5 Choosing the correct cantilever As a general rule gold coated cantilevers have the best photothermal response and lead to the highest amplitudes for the least...

Page 133: ...allow a drive amplitude of 2 mW however the 0 30x filter cube has only 3 mW of DC light power at the cantilever compared to 10 mW of DC light power for the 1 00x filter cube This means that the same c...

Page 134: ...ltern ative to the conventional piezo drive see above l In blueDrive mode the drive amplitude changes from units of V to units of mW to remind the user that the cantilever is driven by blue light powe...

Page 135: ...e box displays the blue spot location on the View Window as shown to the right If the blue spot is not incident on a reflective surface such as the cantilever it may be impossible to see the blue spot...

Page 136: ...SPM User Guide l Right clicking on a partiular location of the View Window and clicking Blue SpotOn auto matically moves the blue spot to that location 16 blueDrive photothermal excitation Page 136 o...

Page 137: ...s shown on the right l For hollow triangular cantilevers the blue spot should be placed at the base of either of the can tilever legs Although it is not necessary to fine tune the blue spot location i...

Page 138: ...before imaging Taking a thermal and fitting it to set the drive frequency to the cantilever resonance may suffice That being said cantilever tunes are very useful in determining whether everything is...

Page 139: ...Part V Tutorials and Workflows This part of the user guide provides tutorials for typical tasks and workflow examples built from the individual tuorials...

Page 140: ...PM User Guide 17 17 Tutorials Introduction The following sections provide task oriented procedures that cover typical setup and data acquis ition using the Cypher SPM Page 140 of 243 17 Tutorials intr...

Page 141: ...knob on the Cypher clock wise and hold it until the cantilever holder is far from the sample or is at its upper limit of travel If you turn the knob counterclockwise you will lower the cantilever hol...

Page 142: ...when it is about halfway out l If you continue pulling the scanner at some point you will feel resistance and should pull no further 5 Familiarize yourself with the sample area l While it may look sol...

Page 143: ...wise should be enough do not completely remove the screw l Replace the tool in its storage place 7 Remove the cantilever holder by holding the tab with the circular recess and pulling straight out tow...

Page 144: ...l Cantilever You will need the chosen cantilever for the experiment you are preparing Every Cypher SPM ships with a package of AC160s Changing sta tion The correct changing station for your cantilever...

Page 145: ...abeled Air there is also a Droplet changing station for the droplet holder l A low power binocular dissection stereoscope with light source can be useful for some of the following steps Tip Cleaning t...

Page 146: ...ining clip l Remove the cantilever and release pressure on the station l Inspect the cantilever area for tiny silicon grit and blow clean with compressed air if necessary 6 Select the new cantilever l...

Page 147: ...e on the chan ging station while still gripping the cantilever chip with tweezers This prevents misalignment caused by the cantilever chip sticking to the tweezers 8 Check cantilever alignment l A pro...

Page 148: ...tandard scanner on page 144 Tools Part 290 102 Tweezers with curved tips Part 290 139 1 16 ball head wrench 1 Rotate the Engage Control knob clockwise until it reaches its upper limit of travel If you...

Page 149: ...ing the tool in the chassis to the left of the scan ner l Do not completely remove the screw 4 Tighten the cantilever holder l Use the ball headed wrench to gently tighten the screw that clamps the ca...

Page 150: ...that the green correction collar on the object ive is set properly l For example for the air cantilever holder set the cor rection collar to 0 7 Close the enclosure door and latch it Page 150 of 243 2...

Page 151: ...on the Cypher clock wise and hold it until the cantilever holder is far from the sample or is at its upper limit of travel If you turn the knob counterclockwise you will lower the cantilever holder ri...

Page 152: ...l If you continue pulling the scanner at some point you will feel resistance and should pull no further 5 Familiarize yourself with the sample area While it may look solid the scanner stage moves the...

Page 153: ...nector l Notice the screws come away from the cutouts in the cantilever holder 2 8 Remove the cantilever holder l After the board has cleared the connector carefully wiggle the cantilever holder up an...

Page 154: ...t the cantilever holder l Identify the correct cantilever holder for your exper iment l This tutorial uses the standard gas holder part 901 758 Tip To learn more about cantilever holders for the envir...

Page 155: ...cantilever holder 5 Select the new cantilever l Select a new cantilever and pick it up with tweezers l Close the box Ruining 1k of levers by putting your hand on an open box is not unheard of l If yo...

Page 156: ...tilever holder window Do not nudge the end of the chip as you risk damaging the cantilever l It helps to do this at least once under a binocular ste reo microscope 7 Secure the new cantilever l Using...

Page 157: ...anging the cantilever on an environmental scanner on page 154 1 Rotate the Engage Control knob clockwise until it reaches its upper limit of travel If you do not raise the stage you will crash the can...

Page 158: ...ring will offer some resistance When the can tilever holder is firmly seated you will feel a hard stop as metal parts make contact 5 Rotate the cantilever holder clockwise until you feel a hard stop...

Page 159: ...is necessary for best per formance l Maintain pressure on scanner and press the lever at the right downward to lock the scanner into place 8 Check the correction collar l Check that the green correcti...

Page 160: ...on the sample surface This task comprises three parts 1 Bringing the cantilever tip to the sample 2 Tuning the cantilever and setting scan parameters 3 Landing the tip 24 1 Bringing the cantilever cl...

Page 161: ...e Approach controls may lead to serious damage to the Cypher 7 Go to last known Tip Focus l On the Engage panel hit Focus on Tip This will move the objective lens to the last known pos ition where the...

Page 162: ...rrows are slow double arrows are fast l Click Set next to Focus on Tip button on the right blue Important The cantilever is at an 11 angle and the whole lever cannot be in focus at once Bring the end...

Page 163: ...ce in the image to the right decimation 1 and zoom 2 See 10 for more information Fun Now that you have stored tip and sample position you can repeatedly click on the buttons Focus on Sample and Focus...

Page 164: ...osition so that it is near the end of the cantilever using the controls in the upper left hand corner of the video panel Note that a decrease in Sum Signal indicates that light is spilling off the can...

Page 165: ...Cypher SPM User Guide Sub Figure a First view of new cantilever 24 Engaging the cantilever on the sample Page 165 of 243...

Page 166: ...Cypher SPM User Guide Sub Figure b Cantilever found and roughly centered on screen 8 Page 166 of 243 24 Engaging the cantilever on the sample...

Page 167: ...Cypher SPM User Guide Sub Figure c Cantilever in focus See 9 24 Engaging the cantilever on the sample Page 167 of 243...

Page 168: ...Cypher SPM User Guide Sub Figure d Optimize Resolution See 10 Figure 4 1 Finding the cantilever and optimizing the video Page 168 of 243 24 Engaging the cantilever on the sample...

Page 169: ...Cypher SPM User Guide Sub Figure a Cantilever Zoom See 10 24 Engaging the cantilever on the sample Page 169 of 243...

Page 170: ...Cypher SPM User Guide Sub Figure b Laser Spot ON See 11 Page 170 of 243 24 Engaging the cantilever on the sample...

Page 171: ...Cypher SPM User Guide Sub Figure c Spot On by right clicking See 11 24 Engaging the cantilever on the sample Page 171 of 243...

Page 172: ...Cypher SPM User Guide Sub Figure d Laser spot on the lever See 12 Figure 4 2 Various methods for aligning the laser spot onto the cantilever Page 172 of 243 24 Engaging the cantilever on the sample...

Page 173: ...Cypher SPM User Guide Sub Figure a Offset 24 Engaging the cantilever on the sample Page 173 of 243...

Page 174: ...Cypher SPM User Guide Sub Figure b The focus offset is centered and the optical image is confocal with the focused laser Page 174 of 243 24 Engaging the cantilever on the sample...

Page 175: ...ne result l The resonance curve should peak around 300kHz l The relevant results are automatically stored After inspecting that the amplitude and phase curves look clean you can close the graph HINT C...

Page 176: ...Cypher SPM User Guide Sub Figure a Master Panel Main Tab Page 176 of 243 24 Engaging the cantilever on the sample...

Page 177: ...ding the tip The preceding sections have left the tip vibrating about 50 microns above the sample surface It s time to land 1 Start final tip approach l Locate the Engage Panel l Click on the Start Ti...

Page 178: ...ting cantilever to reduce its amplitude to the set point One final time the sample is fully retracted and the cantilever is motored down just enough so that when the sample is brought back up it will...

Page 179: ...not be in focus The fix is extra optics just before the video camera Adjust the focus ring at the center in the photo on the right on the view system until the sample is in focus Of course the cantile...

Page 180: ...d on the previous section it is assumed that l The cantilever tip is on the surface or was just disengaged from the surface l The laser is aligned on the cantilever and the photo detector difference d...

Page 181: ...3 Images are saved to disk automatically at the end of every image if you leave the Save Images check box selected near the lower left hand corner of the Master Panel in 1 1 25 2 4 4 2 Start Imaging...

Page 182: ...wo as being quite different this is an indication that imaging parameters need to be adjusted 4 Nomenclature In the previous image the tip is not following the surface As the blue trace shows left to...

Page 183: ...r Any parameter with a radio button next to it can be changed during a scan when it is activated looks like black green dot in circle with the Hamster The Hamster gives digital control with analog fee...

Page 184: ...eper l Keep increasing the Integral Gain until suddenly the trace and retrace start to oscillate wildly a phenomenon called ringing The feedback loop is now unstable and the i gain needs to be lowered...

Page 185: ...in alignment Be sure to avoid accidentally moving the tip and remember that the smaller arrow buttons in the upper left hand corner of the Video Panel are set to the cantilever rather than to the sam...

Page 186: ...Cypher SPM User Guide Sub Figure a Before the Move Page 186 of 243 25 AC mode imaging...

Page 187: ...Cypher SPM User Guide Sub Figure b After the Move Figure 4 5 Moving a Sample After a Scan 25 AC mode imaging Page 187 of 243...

Page 188: ...ed Note Last Scan will change to Undo Last if clicked Clicking Undo Last will cause the software to keep imaging with current parameters 2 Emergency Stopping Procedures l Clicking the Stop button on t...

Page 189: ...Cypher SPM User Guide 26 26 Contact Mode Imaging In Air 26 Contact mode imaging in air Page 189 of 243...

Page 190: ...erence the ORCA holder will not work for meas urement techniques where the tip needs to be biased Note EFM Electric Force Microscopy Surface Potential SKPM Kelvin Probe Microscopy PFM Piezoelectric Fo...

Page 191: ...mechanically to a sample puck as you would with any sample The ORCA kit comes with a practice sample of graphite HOPG mounted to a steel puck A small magnet is attached to the sample puck to provide a...

Page 192: ...ance to keep the OR CA amplifier from saturating An example of this is the HOPG sample provided in the kit l Substitute the Bias lead with a bias lead including a resistor Note The ORCA holder kit inc...

Page 193: ...ch as 901 767 see Visual iden tification of ES cantilever holders on page 54 The gas cell body 901 746 see Gas cell on page 62 The gas cell cable 448 137 see Gas cell parts list on page 63 The Electri...

Page 194: ...magnet would hit the cantilever holder before the cantilever engaged on the sample Attempting to starting a tip approach on a non flat sample may cause serious damage to your can tilever holder and sa...

Page 195: ...template from the Mode Master The Orca template is located under Elec trical Orca l Note the ORCA cantilever holder must be first attached to the AFM to load this template Load a conductive cantileve...

Page 196: ...nd Current 2 Select Current as one of the channel Inputs under the Master Channel Panel AFM Controls Master Channel Panel Ctrl 7 For the Dual gain ORCA holder you will have two channels instead Curren...

Page 197: ...he Sample Voltage parameter on the DoIV panel When you first engage on the sample you should start with a light contact force or low Setpoint but may find that you will have to slowly increase the for...

Page 198: ...ach of the cantilever tip toward the sample It is designed for a Cypher SPM equipped with the standard scanner and a standard spot SLD or laser module see Types of light tube and how to identify them...

Page 199: ...sion with the sample and damage to the scanner l For safety reasons we recommend making motor moves with the door closed Beware of pinch points 2 Lift the door latch and open the enclosure door 3 Unlo...

Page 200: ...ly up to 40 m 6 Release the cantilever holder l Loosen the screw clamping the cantilever holder One turn counterclockwise should be enough l Replace the tool in its storage place hole in the chassis t...

Page 201: ...tion is labeled Air there is also a Droplet changing station for the droplet holder l A low power binocular dissection stereoscope with light source can be useful for some of the following steps Tip C...

Page 202: ...he can tilever retaining clip l Remove the cantilever and release pressure on the station l Inspect the cantilever area for tiny silicon grit and blow clean with compressed air if necessary 13 Select...

Page 203: ...r a binocular ste reo microscope 16 Prepare the scanner and load the sample l Remove any sample that may be present on the scan ner l Wipe the scanning stage see step 5 clean with a soft cloth Any dus...

Page 204: ...ner in place l Gently slide the scanner back into the chassis l Push the lever at the right of the scanner downward to secure the scanner in place 20 Check correction collar l Check that the green cor...

Page 205: ...nced user watching and providing advice Note If you own the standard scanner please follow the tutorial in Loading the cantilever and sample on a standard scanner This tutorial covers sample and canti...

Page 206: ...mit of travel If you turn the knob counterclockwise you will lower the cantilever holder risking collision with the sample and damage to the scanner l For safety reasons we recommend making motor move...

Page 207: ...While it may look solid the scanner stage moves the sample in the X Y and Z directions imper ceptibly up to 40 m Cantilever holder in place Cantilever holder removed 6 Release the cantilever holder l...

Page 208: ...e the cantilever holder comes is the cantilever holder O ring sliding out from the cell body Tip After the O ring clears the cell body be ready for the resistance to drop suddenly Keep a firm grip on...

Page 209: ...e half turn l Do not remove the screw completely If you do refer to Attaching the ES cantilever clip on page 58 13 If a cantilever has already been loaded use tweezers to remove it from the cantilever...

Page 210: ...y centered in the cantilever holder window Do not nudge the end of the chip as you risk damaging the cantilever l It helps to do this at least once under a binocular ste reo microscope 16 Secure the n...

Page 211: ...vent the sample disc from seating properly 19 Replace with new sample l Use Platypus tweezers part 290 165 to place new sample on sample stage l For this tutorial use the Asylum Research calibration g...

Page 212: ...body l The O ring will offer some resistance When the can tilever holder is firmly seated you will feel a hard stop as metal parts make contact 23 Rotate the cantilever holder clockwise until you fee...

Page 213: ...scan ner and the chassis This is necessary for best per formance l Maintain pressure on scanner and press the lever at the right downward to lock the scanner into place 26 Check the correction collar...

Page 214: ...he Cypher SPM equipped with the standard scanner It contains a set of steps that will teach a new user with a basic under standing of AFM operation how to obtain an AC mode topography image in air The...

Page 215: ...the environmental scanner on the previous pageAC mode imaging in air with the standard scanner on the previous page The following assumptions have been made l You understand the aspects of running thi...

Page 216: ...Part VI Shipping and Maintenance This part of the user guide covers moving and shipping the instrument...

Page 217: ...return please use this guide as a reminder 31 2 Moving what does it mean Move a few inches Grip the AFM by its base and slide it around on your workbench by a few inches DO NOT LIFT If you have to li...

Page 218: ...puter 5 Power off the AFM controller 6 Unplug all cables leading to the AFM enclosure and place them aside keeping them together Never lift the AFM enclosure with the instrument inside Don t try to mo...

Page 219: ...is best to raise the stage now to make those later actions possible 2 Lift the door latch and open the enclosure door 3 Power off the ARC2 l Turn the ARC2 off before proceeding l Press the button as s...

Page 220: ...you first place the scanner right in front of the AFM system and then grip the scanner again to move it elsewhere If the scanner is dropped it might become irre parably damaged 6 Detach the scanner fr...

Page 221: ...ntilever holder from the scanner stage as follows l For a standard scanner see steps 5 7 of Loading the cantilever and sample on a standard scanner on page 198 l For an environmental scanner see steps...

Page 222: ...around inside its enclosure Be especially careful while removing and replacing the cover and in general any time the cover is missing The Cypher SPM is heavy but it can slide around If it does slide a...

Page 223: ...acket l Take out two screws on the bracket in front of the view system l After removing both screws pull this piece out and set aside 6 Remove backside screws l Remove all 4 screws from back edge of t...

Page 224: ...y one screw 8 Unscrew right door top l Unscrew right door top inside screws and set aside l SCREW INFO 8 32 x BHCS SS Note There may be only one screw 9 Unscrew left door top l Unscrew left door top i...

Page 225: ...closure on page 233 31 7 Backpack removal and packaging Call or otherwise contact Asylum Research and talk to us before moving anything Chances are that with a few phone calls and the help of this cha...

Page 226: ...rom inside the chassis nearest the backpack screws you just loosened 3 Disconnect the ground cable l The cable is yellow and green l Pull apart the pink connectors 4 Release the pass through board fro...

Page 227: ...face Do not place the backpack on its tall side If it falls over you can cause severe damage 7 Replace the foam removed in step 2 8 Tape the ground cable to the backpack itself Kapton tape is preferre...

Page 228: ...218 Enclosure top removal see Removing the enclosure top cover on page 222 Backpack removal see Backpack removal and packaging on page 225 1 Undo the HDMI cable from the back of the chassis l Slide th...

Page 229: ...surface 4 Undo the 4 petal clamping knob on the chassis head l Identify the knob l Turn only 1 4 turn counterclockwise 5 Remove the light cube from the head l Disconnect the light cube mating cable l...

Page 230: ...ut lens tissue and Kapton tape over the hot mirror and wrap tape around to cover the side port hole 9 Kapton tape the ground cable l It is the green and yellow cable on back side of chassis l Tape it...

Page 231: ...ever l Tape down the scanner connector cable so it does not move in shipment 12 Prepare the shipping guard mullet Note There are variations of the shipping guard l Ensure that five pieces of foam tape...

Page 232: ...4 or 1 2 BHCS SS depending on which guard you have 15 Secure bottom back screws l 2 screws need to go in the bottom back l Use 1 8 hex wrench L key part 290 119 l Include a nut on each screw The nut...

Page 233: ...le when shipping on crate or pallet 31 9 Packaging the enclosure Call or otherwise contact Asylum Research and talk to us before moving anything Chances are that with a few phone calls and the help of...

Page 234: ...enclosure back on by setting down its rear first and then using the dowels and the two holes in the top front to guide you l When the roof is on completely shut the door on the right side carefully 3...

Page 235: ...NFO 8 32 x 1 2 BHCS SS 6 Replace all four screws from the back edge of the enclosure top plate l They are visible above the backpack l SCREW INFO 8 32 x 1 4 BHCS SS 7 Replace the bridge in the front t...

Page 236: ...ace enclosure in the box l Carefully lift the enclosure and place in the bottom of the box Two people should lift the enclosure because it weighs approximately 90 lbs l Ensure the enclosure sits on to...

Page 237: ...he job yourself Not calling us will likely result in instrument damage costly repairs and downtime and the voiding of your war ranty Materials Part 991 005 Cardboard carton 20 x 14 x 8 Part 990 037 Fo...

Page 238: ...side of the View module l The cable is black and is similar to a USB cable 3 Remove the View module l Unscrew and remove the two screws from the top bridge of the View module l Use the 3 16 ball end...

Page 239: ...eeping 7 Remove the objective l Locate the clear plastic objective container with the black cap Remove the black cap and have it sitting ready l Remove the objective from the chassis by unscrew ing it...

Page 240: ...module foam parallel to the view module itself 10 Put on the top foam make sure the square hole lines up with the corner where the view mod ule piece sticks up 11 Bag the pass through board in an ant...

Page 241: ...gging put into the plastic case Part 279 055 l Open and close the case with yellow plastic clips C l Put the light cube case inside any slot of the access ory foam 2 Pack the cantilever changing stati...

Page 242: ...Put any additional accessories for this specific order in the foam Note Smaller items should already have been placed in the Cypher accessory kit 31 12 Packaging computers and monitors Pack computers...

Page 243: ...ox 5 2 Crate or pallet 46 21 Standard Total 383 174 Options affecting shipping weight Item Weight lbs kg TS 150 48 22 30 monitor 31 14 CYPHER ATC 25 11 Crate dimensions The standard crate dimensions a...

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