Micromass Quattro Ultima, User Manual

Page: 19 / 227

The Daughter Ion Spectrum
This is the most commonly used MS-MS scan mode. Typical applications are:
•
Structural elucidation (for example peptide sequencing).
•
Method development for MRM screening studies:
Identification of daughter ions for use in MRM “transitions”.
Optimisation of CID tuning conditions to maximise the yield of a specific
daughter ion to be used in MRM analysis.
Example:
Daughters of the specific parent at
m
609 from reserpine in electrospray
positive ion mode.
The result:
Instrument Description
Page 19
Quattro Ultima
User's Guide
MS1
static at m/z 609
(parent mass)
MS2
scanning from
m/z 100 to 650
Collision Cell
RF only
(pass all masses)