Micromass Quattro Ultima, User Manual

Page: 164 / 227

Glass Capillary Option
Warning: Do not touch the sharp end of the capillary. As well as the risk of
injury by a sliver of glass, the needle becomes inoperable.
Caution: The capillaries are extremely fragile and must be handled with great
care. Always handle using the square end of the capillary.
With the stage rotated outwards, unscrew the union from the end of the
assembly.
Carefully remove the capillary from
its case by lifting vertically while
pressing down on the foam with two
fingers.
Over the blunt end of the capillary,
pass the knurled nut, approximately
5mm of conductive elastomer and
finally the union.
Tighten the nut (finger tight is
sufficient) so that 5mm of glass
capillary is protruding from the end
of it. This distance is measured from the end of the nut to the shoulder of the
glass capillary.
Load sample into the capillary using either a
fused silica syringe needle or a gel loader tip.
Screw the holder back into the assembly - finger
tight is sufficient.
Ensure that Capillary is set to 0V on the tune
page.
Rotate the stage back into the interface using the
stop and handle.
Manoeuvre the stage so that the microscope or
camera can view the capillary tip.
Using a 10ml plastic syringe or a regulated gas
supply, apply pressure to the back of the tip until
a drop of liquid is seen. Remove the back
pressure.
On the tune page, select Gas , Gas to turn on the nitrogen.
Select .
Nanoflow Electrospray
Page 164
Quattro Ultima
User's Guide
Foam
Capillary
Glass
Capillary
Blue
Conductive
Elastomer
PTFE
"Back Pressure"
Tubing
Ferrule
5mm
Knurled Nut
Union