Micromass Quattro Ultima, User Manual

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LC-MS Sensitivity Enhancement
The sensitivity of a LC-MS analysis can be increased or optimised in a number of
ways, by alterations to both the LC operation and the MS operation.
In the LC area some examples include the use of high resolution columns and columns
with fully end capped packings. For target compound analysis, techniques such as
trace enrichment, coupled column chromatography, or phase system switching can
have enormous benefits.
Similarly, the mass spectrometer sensitivity can often be significantly increased, for
instance by narrow mass scanning or by single ion recording (SIR) techniques.
Careful choice of the solvent, and solvent additives or modifiers may also prove
important.
Electrospray
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Quattro Ultima
User's Guide