Micromass Quattro Ultima, User Manual

Page: 166 / 227

Nano-LC Option
Installation
With the sprayer assembly removed
from the stage:
Cut approximately 25mm of the
red stripe peek tubing and, using
the plug cap and a Valco nut, set a
ferrule to the correct position on
the tubing.
At this stage the ferrule is required
only to grip the tubing lightly, and
should not be too tight.
Cut the peek such that 10mm of
the peek protrudes from the back
of the ferrule.
Thread approximately 70mm of
the 90 micron o.d. fused silica
through the new fitting.
Ensure that the fused silica is flush
with the peek sleeve.
Again using the plug cap, tighten
the nut further to ensure that the
fused silica is gripped. Some force
may be required to do this.
Remove the sleeved fused silica
from the plug cap and remove the
Valco nut.
Place an O ring onto the peek tube,
using tweezers if necessary.
The O ring is required to seal the
region between the ferrule and the end of the thread on the nano-LC chamber.
Thread the sleeved fused silica through the nano-LC chamber.
Rotate the microvolume union in the body such that the ferrule seat is aligned
correctly.
Insert the chamber into the nano-LC body and tighten using a pair of spanners.
Nanoflow Electrospray
Page 166
Quattro Ultima
User's Guide
1mm
From Injector
(or Column
Attached Directly)
Make-up
Flow
Only
(3-way
Insert
Required)
Nebuliser
Gas
Nano-LC
Body
Chamber
Nebulising
Tip
Red Stripe
Peek Tubing
O Ring
90µm Fused
Silica
Valco
Ferrule
Microvolume
Insert