Micromass Quattro Ultima, User Manual

Page: 132 / 227

Scanning Calibration and Scan Speed Compensation
Acquiring Data
To complete the calibration of the instrument two further data files must be acquired.
Both files are acquired in scanning mode over the same mass range, one at the slowest
speed required for scanning acquisitions and one at the fastest speed. Once these files
have been acquired and used for calibration then data may be acquired anywhere
within the mass range at any scan speed between the values used for the two sets of
data. These data do not have to be acquired through the calibration dialog box, they
can be acquired using the normal scan setup and then accessed from the calibration
dialog box as described below.
The recommended scan speed for the scanning calibration is 100 amu/sec.
Set
Scan From
to 80 amu and
Scan To
to 1000 amu.
Set Scan Time to 9.2 sec and Inter Scan Delay to 0.1 sec.
Select
Continuum
as the
Data Type
.
Although Continuum is recommended centroided data may be used.
Set Run Duration to 2.0 minutes.
This allows time to start the acquisition, inject the reference solution and
acquire several scans. With a solvent flow rate of 200 µl/min and a 50 µl loop in
line, an injection of reference solution lasts approximately 15 seconds allowing
at least one full scan of useful data to be acquired.
Choose any filename for the data.
The filename SCNMS1, the name used during an automatic calibration, is valid.
Start the acquisition and inject the reference solution.
Mass Calibration
Page 132
Quattro Ultima
User's Guide