Micromass Quattro Ultima, User Manual

Page: 172 / 227

Obtaining a Beam
Ensure that the corona discharge pin is fitted and connected as described in
Routine Procedures, Preparation for APcI Operation.
Ensure that the APcI probe is fitted as described above, that the desolvation gas
tube is connected to the front panel, and that the purge gas outlet is plugged.
If necessary, change the ionisation mode using the Ion Mode command.
The top line of the tune page indicates the current ionisation mode.
Set Source Temp to 130°C.
Set
APcI Probe Temp
to 20°C with no liquid flow and the nitrogen off.
Set Corona to 2µA and Cone to 50V.
When Source Temp reaches 130°C:
Select
Gas
,
Gas
to switch on the nitrogen gas.
Using the valves on the front of the instrument, adjust Desolvation Gas to
150 litres/hour and set Nebuliser Gas to its maximum setting.
To monitor the flow rate, select the
Source
window on the tune page and
observe the Gas Flows readback window.
Select one of the peak display boxes and set
Mass
to 50 and
Span
to 90.
Select .
Increase Gain on the peak display
box in the range 1 to 20 until peaks
become clearly visible.
Set
APcI Probe Temp
to 500°C.
When APcI Probe Temp reaches
500°C:
Start the LC pump at a flow of
1 ml/min.
Adjust the probe’s in / out position
so that it is fully retracted.
Adjust the probe’s sideways
position so that the spray is directed
approximately at the midpoint between the corona pin and the sample cone.
Atmospheric Pressure Chemical Ionisation
Page 172
Quattro Ultima
User's Guide
In / Out
Probe
Adjustment
Sideways
Probe
Adjustment