
9.7 Predefined methods and phases
Introduction
A predefined method contains a set of phases, each phase reflecting a specific stage of a chromatog-
raphy or maintenance run. You can select additional phases from the phase libraries and add these
to an existing method, or remove phases that are not required.
The predefined purification methods have default values with suitable running conditions for the
chosen column type such as flow and pressure limits. Other settings (e.g., sample application technique,
sample volume, elution profile and fractionation) are set on the Phase Properties pane in the appropriate
phases.
This section describes the predefined methods and phases.
A method is built up by a number of phases. Each phase represents a major process step in the method,
for example, equilibration or elution. Predefined methods, that include all the phases necessary to run
the system, are available for different chromatography techniques and also for system cleaning.
This sections contains the following subsections:
See page
Section
9.7.1 Predefined purification methods
9.7.2 Predefined maintenance methods
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ÄKTA pure User Manual 29119969 AB
9 Reference information
9.7 Predefined methods and phases
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