GE AKTApure, User Manual, Page: 312 / 522

7.7.4 Calibrate the UV monitors
Automatic calibration of the UV monitor U9-M
The wavelength is automatically calibrated every time the instrument is switched on. If the instrument
has been switched on for a couple of days, and the ambient temperature and/or the humidity has
changed, restart the instrument using the power switch to calibrate the UV monitor.
Calibration of the UV monitor U9-L flow cell length
The path length in the UV flow cell or the UV 2nd flow cell might differ from the nominal length, which
leads to incorrect results in the calculation of protein concentration in the eluate. To achive normalized
absorbance, the path length in the UV flow cell or the UV 2nd flow cell must be calibrated. The calibration
procedure that is described below is the same for both UV flow cell and the UV 2nd flow cell, unless
otherwise stated.
The flow cell path length must be registered or updated in UNICORN, when the flow cell is re-
placed.
Note:
Equipment needed
To perform the calibration, a calibration kit containing test solutions, syringes and accessories is
needed. A specified kit is available for each cell length.
Then use calibration kit... If using a UV flow cell with
the theoretical path
length...
UV-900 2 mm calibration kit (Product Code 18-6324-02) 2 mm
UV-900 5 mm calibration kit (Product Code 18-6324-04) 5 mm
To calculate the real path length of the UV flow cell, use the following software:
• UV-900 cell calibration Excel-file (Product Code 18-6324-06)
Prepare for calibration
Follow the instructions below to prepare for the calibration of the UV monitor U9-L.
Action Step
Ensure that the flow restrictor is inline in the flow path after the UV flow cell. 1
Mount the union Luer female/1/16” male, included in the test kit, in the upper inlet of the
UV flow cell. 2
Open the software UV-900 cell calibration Excel-file. 3
312 ÄKTA pure User Manual 29119969 AB
7 Maintenance
7.7 Calibration procedures
7.7.4 Calibrate the UV monitors