GE AKTApure, User Manual, Page: 192 / 522

Action Step
Load sample into the sample loop. To avoid sample loss due to siphoning, leave the syringe
in the port until the sample has been injected onto the column during the run.
Tip:
It is recommended to overfill the loop to make sure that the loop is completely filled. Excess
of sample will leave the valve through port W1.
7
Fill the sample loop using the Sample pump
Most often the sample loop is filled using a syringe. However, to fill it using the sample pump, follow
the instructions for filling the Superloop, see Fill the Superloop using the Sample pump, on page 185.
Note: • It is not necessary to set the Alarm Sample Pressure when filling a sample loop.
• It is recommended to overload the loop to make sure that the loop is completely
filled.
• Set Flow rate to an appropriate value for the loop size.
• After loading, plug the Syr port on the Injection valve with a Stop plug.
Fill the sample loop using System pump A
Most often the sample loop is filled using a syringe. However, to fill it using System pump A, follow the
instructions for filling the Superloop, see Fill the Superloop using System pump A, on page 187.
Empty the loop
General considerations
During the method run, the sample is automatically injected onto the column. The loop is emptied and
washed out using buffer from the system pumps. The total buffer volume to be used for emptying and
washing the loop is set in the Method Editor.
Using a sample loop connected to Loop valve V9-L or V9H-L
For maximum reproducibility, use complete loop fill when loading the loop, that is, overfill the loop with
a sample volume of up to 3-5 times the volume of the loop. For minimum sample loss, use partial loop
fill, that is, fill only up to 50% of the loop volume. Empty the loop with 3-5 times the volume of the loop.
Volume used to empty a loop connected to Loop valve V9-L or V9H-L
To minimize the risk for carry over and to make sure that the complete sample volume reaches the
column, the loop should be emptied with an excess of buffer. The tubing between the Loop valve port
E and the Injection valve port E holds a small volume. If the loop is emptied with a volume equal to, or
less than the loop volume this needs to be taken into account. It is also important to use a low flow
rate to ensure that the correct volume is added to the column when injecting a small volume.
Partially emptying the loops that are attached to Loop valve V9-L or V9H-L can increase the
risk for carry over from one loop position to the next.
Note:
192 ÄKTA pure User Manual 29119969 AB
5 Operation
5.7 Sample application
5.7.3 Sample application using a sample loop