GE AKTApure, User Manual, Page: 445 / 522

9.7.2 Predefined maintenance methods
A number of predefined methods for preparation and cleaning are available. These maintenance
methods are used to prepare the system, clean the system, and to fill the system with storage solution.
The table below describes the available predefined maintenance methods.
Included phases Principle Predefined maintenance
method
The column is filled with a cleaning solution. Se-
lect inlet positions. Enter the solution identity,
volume, flow rate and incubation time. By adding
steps, several cleaning solutions can be used.
Suggestions for cleaning steps are available for
a number of column types. Column CIP
After equilibration of the column, sample is in-
jected via a loop and eluted isocratically. A non-
adsorbing sample like acetone or salt should be
used. After the run, calculate column perfor-
mance in the Evaluation module. The efficiency
of the column is determined in terms of height
equivalent to a theoretical plate (HETP), and the
peak asymmetry factor (A
s
). The result is logged
in the column logbook.
Column Performance Test
The column is filled with buffer solution. Select
inlet positions. Enter the solution identity, vol-
ume, flow rate and incubation time. By adding
steps, several preparation solutions can be used. Column Preparation
Packs AxiChrom™ columns, with a predeter-
mined column type, by motorized power or by
a flow of hydraulic liquid that pushes the adaptor
down. The user initiates the start of compression
at the exact point when the adapter reaches the
consolidated bed surface. The adapter compress-
es the bed according to the packing factor or
target bed height as selected. Two Column Per-
formance Test phases (upflow and/or downflow)
can be performed after the AxiChrom column
has been packed. Intelligent Packing
Only available for some types of AxiChrom
columns.
Note:
Intelligent packing is also available for systems
that use wizard generated methods.
ÄKTA pure User Manual 29119969 AB 445
9 Reference information
9.7 Predefined methods and phases
9.7.2 Predefined maintenance methods