GE AKTApure, User Manual, Page: 237 / 522

6.6 Fraction collector F9-C test
Method description
The Fraction Collector Test checks the functionality of Fraction collector F9-C.
The method run takes approximately 6 minutes.
Required configuration
The core components of ÄKTA pure, an outlet valve and a correctly installed Fraction collector F9-C
are required to run the test.
Required material
The following materials are required:
• Distilled water
• Syringe, 25-30 ml
• Two deep well cassettes
• Two 96 deep well plates for collecting the fractions
Prepare the test
Follow the instructions below to prepare the system before method start.
Action Step
Immerse waste tubing W in a waste container 1
Immerse inlet tubing A1 in distilled water. 2
Prime inlet tubing A1 and purge System pump A. See Section 5.4.1 System pumps, on
page 162. No air shall be left in the pump or tubing. 3
Place two 96 deep well plates in positions 1 and 6 in the fraction collector. Make sure
that no other cassettes are present in the fraction collector during the run. 4
In the System Control module, select System:Settings. 5
Result: The System Settings dialog opens.
In the System Settings dialog: 6
• Select Fraction collection:Fractionation settings.
• In the Fraction mode field, select Automatic.
• In the Fractionation order field, select Row-Automaticby-Row.
• Click OK.
ÄKTA pure User Manual 29119969 AB 237
6 Performance tests
6.6 Fraction collector F9-C test