GE AKTApure, User Manual, Page: 448 / 522

Description Phase Name
Equilibrates the column before purification, or re-equilibrates the column after
purification. Equilibration
Applies sample to the column. Defines the sample application technique, the
sample volume, and the handling of flowthrough. Sample Application
Washes out unbound sample after sample application or removes strongly
bound proteins after elution. Column Wash
Elutes the sample from the column. Defines parameters for the elution and
fractionation settings. Elution
Prepares the column before use by removing the storage solution and equili-
brating the column. By adding steps, several preparation solutions can be
used sequentially. Column Prepara-
tion
Cleans the column after purification runs by rinsing the column with a
cleaning solution to remove nonspecifically bound proteins. By adding steps,
several cleaning solutions can be used sequentially. Column CIP
Prepares the system before a run by removing storage solution and filling
the system and inlets with buffer solution. One preparation solution is used
per phase. System Prepara-
tion
Cleans the system after purification runs by rinsing the system with a cleaning
solution. One cleaning solution is used per phase. System CIP
Tests the efficiency of a packed column in terms of height equivalent to a
theoretical plate (HETP), and the peak asymmetry factor (A
s
).
Column perfor-
mance test
Is used to manually fill the additional sample application loops mounted on
the loop valve. The filling options are: Manual Loop Fill
• Partial loop fill
• Complete loop fill
Can be added to any method at suitable places. The instructions can help
the user to better organize the graphical output of the results or introduce a
controlled delay in the method run. Miscellaneous
448 ÄKTA pure User Manual 29119969 AB
9 Reference information
9.7 Predefined methods and phases
9.7.3 Predefined phases