GE AKTApure, User Manual, Page: 185 / 522

Action Step
If the loop is connected to: 3
• the Injection valve, continue to step 5.
• the Loop valve, continue to step 4.
In the Process Picture: 4
• Click the Loop valve.
• Select the position the loop is connected to, for example Position 1.
Result: The Loop valve switches to the selected position.
Fill a syringe with sample. 5
Connect the syringe to Injection valve port Syr. 6
Load sample into the Superloop by emptying the syringe into the Injection valve. 7
Disconnect the syringe and plug the Syr port with a Stop plug. 8
Fill the Superloop using the Sample pump
Follow the instruction below to fill the Superloop using the Sample pump.
NOTICE
Glass tube splinter. Make sure to set the sample pressure below the max pressure
of the Superloop before executing a flow in the Manual instructions dialog when the
Superloop is connected.
The Superloop can also be filled as part of a method run, as set in the Sample Application
phase in the Method Editor. For multiple injections, it may be more convenient to fill the Super-
loop once, as described in the instruction below.
Tip:
ÄKTA pure User Manual 29119969 AB 185
5 Operation
5.7 Sample application
5.7.2 Sample application using a Superloop
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