GE AKTApure, User Manual, Page: 496 / 522

Standard delay volumes
The table below lists the delay volumes for standard configurations using the available tubing kits with
and without a pH valve and using tubing of standard length from the outlet valve to the fraction col-
lector. The values are based on the flow cells for UV monitor U9-M. When using U9-L, add 9 µl for the
2 mm flow cell and 4 µl for the 5 mm flow cell. The values for delay volume Monitor to frac F9-C assume
that the internal tubing of Fraction collector F9-C has the same inner diameter as the tubing kit.
Delay volumes ÄKTA pure 25
Delay volume UV monitor to point of collection of eluent Delay vol-
ume pH
valve re-
strictor pH
valve
V9-pH Tubing kit
Delay volume
Monitor to frac
F9-C Delay volume
Monitor to frac
F9-R Delay volume
Monitor to outlet
valve
214 µl 86 µl 66 µl N/A No 0.25
225 µl 96 µl 77 µl 25 µl Yes 0.25
435 µl 205 µl 125 µl N/A No 0.5 (stan-
dard)
462 µl 231 µl 152 µl 48 µl Yes 0.5 (stan-
dard)
803 µl 400 µl 223 µl N/A No 0.75
857 µl 454 µl 277 µl 88 µl Yes 0.75
1319 µl 675 µl 361 µl N/A No 1.0
1410 µl 766 µl 452 µl 143 µl Yes 1.0
Delay volumes ÄKTA pure 150
Delay volume UV monitor to point of collection of eluent Delay vol-
ume pH
valve re-
strictor pH
valve
V9H-pH Tubing kit
Delay volume
Monitor to frac
F9-C Delay volume
Monitor to frac
F9-R Delay volume
Monitor to outlet
valve
508 µl 278 µl 198 µl N/A No 0.5
555 µl 324 µl 245 µl 60 µl Yes 0.5
876 µl 473 µl 296 µl N/A No 0.75 (stan-
dard)
950 µl 547 µl 370 µl 100 µl Yes 0.75 (stan-
dard)
1392 µl 748 µl 434 µl N/A No 1.0
1503 µl 859 µl 545 µl 155 µl Yes 1.0
496 ÄKTA pure User Manual 29119969 AB
9 Reference information
9.13 Delay volumes