GE AKTApure, User Manual, Page: 313 / 522

Action Step
The solution bottles are labelled with the concentration value and the reference ab-
sorbance value for each solution. Enter the concentrations of the solutions in ascending
order into the column UV Test kit Concentration (mg/l). Enter the corresponding ab-
sorbance values into the column UV Test kit Absorbance (AU/cm). 4
Perform the calibration
Follow the instructions below to calibrate UV monitor U9-L and UV monitor U9-L, 2nd.
Action Step
In the System Control module, select Manual:Execute Manual Instructions. 1
In the Manual Instructions dialog:
• Select Pumps:System flow and set the Flow rate to 0.0 ml/min.
• Click Execute.
Result: The absorbance can now be monitored.
Fill one of the supplied syringes with 1.5-2 ml of the first solution (0 mg/l). Ensure that
there are no air bubbles in the syringe. 2
Fit the syringe in the union Luer connector and inject the solution. DO NOT remove the
syringe. 3
Note:
Air trapped in the UV cell causes inaccurate measurements. To avoid introducing air into
the UV cell, gently fill the union Luer up to the edge with test solution that is to be introduced,
using the syringe. Then insert the syringe into the union Luer.
Wait until the monitored absorbance value has stabilized. 4
ÄKTA pure User Manual 29119969 AB 313
7 Maintenance
7.7 Calibration procedures
7.7.4 Calibrate the UV monitors