GE AKTApure, User Manual, Page: 392 / 522

Incident: Leaking outlet check valve.
Actions: Replace the check valve. See Section 7.8.9 Replace the pump head check valves, on page 332.
Incident: One inlet is blocked,
Action: Clean inlet tubing. For example, perform a System CIP.
Remove persistent air bubbles
After purging the pump (see Section 5.4 Prime inlets and purge pump heads, on page 161), check that
all air bubbles have been removed by analyzing the pre-column pressure curve (see examples above).
If the pressure curve indicates that there are still air bubbles present, repeat the purging process. If
the problem persists, follow the instructions below to purge the pump with methanol (see Section 5.4
Prime inlets and purge pump heads, on page 161 for detailed instructions for the purge procedure).
1 Make sure that the pump contains water.
2 Use a syringe to draw 100% methanol into the pump (see Section 5.4 Prime inlets and purge pump
heads, on page 161 for details).
3 Set the pump flow to 2.5 ml/min for ÄKTA pure 25 or 10 ml/min for ÄKTA pure 150.
4 Let the flow run until the disturbances in the pressure curve disappear.
5 Remove the methanol:
• Stop the pump and move the inlet tubing to water. Make sure that no air is introduced into the
system.
• Set the pump flow to 1 ml/min for ÄKTA pure 25 or 5 ml/min for ÄKTA pure 150.
• Run for 5 minutes.
392 ÄKTA pure User Manual 29119969 AB
8 Troubleshooting
8.6 Troubleshooting: Pumps