GE AKTApure, User Manual, Page: 238 / 522

Action Step
In the System Settings dialog: 7
• Select Tubing and Delay volumes.
• Select the instruction Delay volume: Monitor to frac
• Set the correct delay volume, see Section 9.13 Delay volumes, on page 494
• Click OK.
Run and evaluate the test
Follow the instructions described in Section 6.1 General performance test actions, on page 226 to start
and run the performance test.
During the run
• Visually check that the fraction collector wash is performed.
• Several messages will be displayed on the screen during the startup of the run. Read the messages
in the dialog and make sure that necessary preparations have been performed. Click the "Confirm
and Continue" button in the message dialog to proceed with the test.
Evaluate the result
To evaluate the results, do the following:
• Check that correct volumes, 2 ml/well, are collected in the three first wells in row A (A1-A3) of both
plates.
• Check if the fractionation marks in the chromatogram are in accordance with the filled wells. For
further information on delay volumes and fractionation marks, see Delay volume, on page 208.
238 ÄKTA pure User Manual 29119969 AB
6 Performance tests
6.6 Fraction collector F9-C test