GE AKTApure, User Manual, Page: 439 / 522

9.7.1 Predefined purification methods
The Method Editor has predefined methods for different separation techniques. The methods include
a number of relevant phases.
The table below describes the available predefined purification methods and which phases that are
included.
Included phases Principle Predefined purification
method
After equilibration and sample application, the
protein of interest is adsorbed to the column
ligand. After a wash to remove unbound sample,
elution is performed either by using a buffer
containing a competitor to displace the protein
of interest, or by changing the pH or ionic
strength. Finally, the column is re-equilibrated
with start buffer. Affinity Chromatography (AC)
ÄKTA pure User Manual 29119969 AB 439
9 Reference information
9.7 Predefined methods and phases
9.7.1 Predefined purification methods