14
Theoretical Background
Theoretical basis of 2D-LC
2D-LC User Guide
368
Peak Capacity
Peak capacity may be differently defined:
•
As the maximum number of peaks that can be resolved in the available
separation space (
Geometrical Definition
), or
•
As the ratio of the total area of the chromatogram to the area required for the
resolution of any zone (
General Definition
)
Geometrical
Definition
The peak capacity may be defined as the maximum number of peaks that can be
resolved in the available separation space. So peak capacity n
c
is related to the
number of theoretical plates N:
(
depends on the retention time range)
In practice peaks are usually not distributed randomly over the chromatogram
and often overlap. Or in other words: In practice peaks don't fill the available
separation space evenly. This is the reason, why the number of detectable
components of a sample in 1D-LC is relatively small.
2D-LC separation offers an alternative possibility for increasing n
c
: Orthogonal
retention mechanisms generate a separation plane. Thus, the peak capacity in
2D-LC is the product of the peak capacities of the individual columns. Due to
peak broadening in 1
st
and 2
nd
dimension, components in 2D-LC are present as
two-dimensional ellipses on the retention plane.
How to calculate n
c
depends on the method:
•
For comprehensive 2D-LC:
•
For heart-cutting 2D-LC:
L = Separation space for dimension
ab = Area for rectangle circumscribing the ellipse on the separation plane
Содержание 1290 Infinity II 2D-LC Solution
Страница 122: ...5 2D LC Data Acquisition in MassHunter Workstation 11 Instrument Details 2D LC User Guide 122 3 Click Screen 4 Click OK...
Страница 263: ...2D LC User Guide 263 9 Data Analysis GC Image Basic Information 3 Import parameters...
Страница 264: ...9 Data Analysis GC Image Basic Information 2D LC User Guide 264 4 Fit view...
Страница 267: ...2D LC User Guide 267 9 Data Analysis GC Image Basic Information 11 View single 2D chromatograms 12 Select blobs...
