2D-LC User Guide
207
8
Run the System
Run the Experiment
Table 19
Recommended conditions in 2D (HPLC) for multiple heart-cutting
Parameter
Value
2D-LC Valve
MHC with 40 µl sample, Transfer Capillary, ASM Factor No
2
D Column Compartment (MCT)
Column
RRHD Bonus-RP, 2.1x 50 mm, 1.8 µm, 1200 bar (857768-901)
Column temperature
40 °C
Stop time
As pump/No limit
2
D Pump
Heart Cutting (time or peak based)
Mobile Phase A
Water + 0.2 % formic acid
Mobile Phase B
Acetonitrile
Flow Rate
1 mL/min
Idle flow
not used
Stop time
40 min (will not automatically prolonged, if peaks in 2D are not work off)
Post time
6 min
Sampling Table
Start 4.08 min, 13 cuts required (time based or peak based), Cut Size 4.0
Figure 107 Time-based
Figure 108 Peak-based
The Cut-Time (MHC) can vary slightly depending on the configuration and the used hardware.
Содержание 1290 Infinity II 2D-LC Solution
Страница 122: ...5 2D LC Data Acquisition in MassHunter Workstation 11 Instrument Details 2D LC User Guide 122 3 Click Screen 4 Click OK...
Страница 263: ...2D LC User Guide 263 9 Data Analysis GC Image Basic Information 3 Import parameters...
Страница 264: ...9 Data Analysis GC Image Basic Information 2D LC User Guide 264 4 Fit view...
Страница 267: ...2D LC User Guide 267 9 Data Analysis GC Image Basic Information 11 View single 2D chromatograms 12 Select blobs...
