2D-LC User Guide
225
8
Run the System
Run the Experiment
Table 32
Recommended conditions in 1D (HPLC), ASM comprehensive
Parameter
Value
1
D Column Compartment (MCT)
Column
RRHD SB-C18, 2.1x 100 mm, 1.8 µm, 1200 bar (858700-902)
Column temperature
40 °C
Stop time
As pump/No limit
1
D Pump
Mobile Phase A
Water + 0.2 % formic acid
Mobile Phase B
Methanol
Flow Rate
0.1 mL/min
Stop time
40 min
Post time
6 min
Mobile Phase
Gradient:
20 % B 0.00 min
100 % B 50 min
80 % B 7.00 min
Autosampler
Injection Volume
2 µLfor Standard LC
0.5 µL Positive Mode for LCMS
10 µL Negative Mode for LCMS
Injection Needle Wash In Flush Port, 10 s, acetonitrile/water (50/50)
Stop time
As pump/No limit
1
D Detector (DAD)
Diode-array Detector
Signal A
254 nm, Bandwidth 4 nm
Reference Wavelength 360 nm
Reference Bandwidth
100 nm
Peak width
20 Hz
Stop time
Stop time As pump/No limit
Содержание 1290 Infinity II 2D-LC Solution
Страница 122: ...5 2D LC Data Acquisition in MassHunter Workstation 11 Instrument Details 2D LC User Guide 122 3 Click Screen 4 Click OK...
Страница 263: ...2D LC User Guide 263 9 Data Analysis GC Image Basic Information 3 Import parameters...
Страница 264: ...9 Data Analysis GC Image Basic Information 2D LC User Guide 264 4 Fit view...
Страница 267: ...2D LC User Guide 267 9 Data Analysis GC Image Basic Information 11 View single 2D chromatograms 12 Select blobs...
