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8
Run the System
Run the Experiment
2D-LC User Guide
226
Table 33
Recommended conditions in 2D (HPLC) for ASM comprehensive 2D-LC
Parameter
Value
2D-LC Valve
MHC with 40 µl sample, Transfer Capillary, ASM Factor No
2
D Column Compartment (MCT)
Column
RRHD Bonus-RP, 2.1x 50 mm, 1.8 µm, 1200 bar (857768-901)
Column temperature
40 °C
Stop time
As pump/No limit
2
D Pump
Comprehensive
Figure 112 Comprehensive
Mobile Phase A
Water + 0.2 % formic acid
Mobile Phase B
Methanol
Flow Rate
2.5 mL/min
Stop time
40 min (will not automatically prolonged, if peaks in 2D are not work off)
Post time
6 min
2D gradient
Analysis 0.2 min Equilbration 0.1 min
ASM Off
Analytical gradient - shifted gradient shift 1D
25 % B 0.00 min
50 % B 40 min
50 % B 0.20 min
75 % B 40 min
25 % B 5 min
50 % B 5 min
2
D Detector (DAD)
Diode-array
254 nm, Bandwidth 4 nm
Reference Wavelength 360 nm
Reference Bandwidth
100 nm
Peak width
80 Hz
Stop time
As pump/No limit
Содержание 1290 Infinity II 2D-LC Solution
Страница 122: ...5 2D LC Data Acquisition in MassHunter Workstation 11 Instrument Details 2D LC User Guide 122 3 Click Screen 4 Click OK...
Страница 263: ...2D LC User Guide 263 9 Data Analysis GC Image Basic Information 3 Import parameters...
Страница 264: ...9 Data Analysis GC Image Basic Information 2D LC User Guide 264 4 Fit view...
Страница 267: ...2D LC User Guide 267 9 Data Analysis GC Image Basic Information 11 View single 2D chromatograms 12 Select blobs...