Agilent Technologies 1290 Infinity II 2D-LC Solution, Руководство пользователя

Страница: 256 / 395

9 Data Analysis
GC Image Basic Information
2D-LC User Guide 256
GC Image Basic Information
Typically very complex samples are analyzed by comprehensive 2-dimensional
liquid chromatography. The compounds which are often co-eluting form the first
dimension are further separated in the second dimension. With the Agilent 1290
Infinity II 2D-LC Solution always one large data-file spanning the run-time of the
two-dimensional analysis will be acquired. As an example, a 2-dimensional
analysis of a mixture of 26 polyphenolic standard compounds is shown in a one
dimensional data analysis display (
Figure 138 on page 257). Theoretically, the
data can be analyzed with Agilent MassHunter Workstation Software Qualitative
Analysis software.
But for easier data-analysis and better visualization of the comprehensive 2D-LC
data special software is recommended. Agilent recommends GC Image LCxLC
edition Software from GC Image LLC, Nebraska, USA. A trial download can be
found on
www.GCImage.com as well as an online manual. Agilent 2D-LC data
files also including UV spectra and mass spectra data can be directly imported.
This software, with the information of the modulation time, is capable to extract
the data and isolate each second dimension run. Data will be reconstructed in a
two-dimensional display of the retention times. This can be displayed as a
colored 2-dimensional map of compound peaks (
Figure 139 on page 257). After
baseline correction the peaks can be automatically detected by a peak detection
algorithm inherent in the 2D-LC data analysis software (
Figure 140 on page 258).
Since the third dimension is the intensity of the peaks a 3-dimensional plot of the
data is possible (
Figure 141 on page 258). With the given data set further
qualitative and quantitative data analysis is possible.