2D-LC User Guide
223
8
Run the System
Run the Experiment
1
Load method
ASM Multiple Heart-Cutting Checkout
from the 2D-LC data media
and modify the settings for your multiple heart-cutting configuration.
2
Run the method with 2D-LC starter sample, 1 x 2 mL (5190-6895), 1:10 (for
only UV Checkout), 1:100 (for LCMS Checkout), or 1:1000 (for LCMS
Checkout) diluted with Methanol/Water (20/80; v/v) with 0.1 % formic acid.
3
If necessary, subsequently edit or optimize the method.
Min Height
1000 counts
Reference Masses
Positive
121.05087300
922.00979800
Chromatograms
Chrom Type Label Offset Y-Range
TIC TIC 1510000000
TIC TIC 1510000000
Stop time
As pump/No limit
Table 30
Recommended conditions in 2D (LC-MS)
Parameter
Value
NOTE
Adjust the ASM split ratio
To optimize the ASM split ratio of the method either for highest resolution
(strong dilution), or lowest cycle time (weak dilution), different ASM capillaries
are available.
The checkout method uses ASM factor 3, see
Содержание 1290 Infinity II 2D-LC Solution
Страница 122: ...5 2D LC Data Acquisition in MassHunter Workstation 11 Instrument Details 2D LC User Guide 122 3 Click Screen 4 Click OK...
Страница 263: ...2D LC User Guide 263 9 Data Analysis GC Image Basic Information 3 Import parameters...
Страница 264: ...9 Data Analysis GC Image Basic Information 2D LC User Guide 264 4 Fit view...
Страница 267: ...2D LC User Guide 267 9 Data Analysis GC Image Basic Information 11 View single 2D chromatograms 12 Select blobs...
