2D-LC User Guide
269
9
Data Analysis
GC Image Basic Information
Investigate the effects of using different gradients in
2
D
When combining separation systems with related separation mechanisms in the
first and second dimension (as in RPxRP), orthogonality is limited. As a result,
only a part of the available two-dimensional separation space will be occupied. In
such a case, shifted gradients in the second dimension can be used to enlarge
the accessible two-dimensional separation space.
1
To investigate the effects of using different gradients in the second
dimension, firstly run a comprehensive 2D-LC separation with the same
second dimension gradient from 5 – 95 % B repeated during the whole run.
The
1
D pump method should be set up as during the checkout runs (see
below):
Содержание 1290 Infinity II 2D-LC Solution
Страница 122: ...5 2D LC Data Acquisition in MassHunter Workstation 11 Instrument Details 2D LC User Guide 122 3 Click Screen 4 Click OK...
Страница 263: ...2D LC User Guide 263 9 Data Analysis GC Image Basic Information 3 Import parameters...
Страница 264: ...9 Data Analysis GC Image Basic Information 2D LC User Guide 264 4 Fit view...
Страница 267: ...2D LC User Guide 267 9 Data Analysis GC Image Basic Information 11 View single 2D chromatograms 12 Select blobs...
