2D-LC User Guide
199
8
Run the System
Prepare the Experiment
Prepare the Experiment
Preparation of 4 mL sample (1:100) for LCMS
1
To prepare dilution solvent, add 800 µL methanol to 3200 µL Mobile Phase A.
4000 µL dilution solvent (20 % methanol in mobile phase A) is prepared.
2
To prepare sample (1:100), add 40 µL 2D-LC starter sample to 3960 µL
dilution solvent.
4 mL sample (1:100) is prepared.
A further dilution step to 1:1000 may be useful.
Preparation of 4 mL sample (1:1000) for LCMS
1
To prepare dilution solvent, add 800 µL methanol to 3200 µL Mobile Phase A.
4000 µL dilution solvent (20 % methanol in mobile phase A) is prepared.
2
To prepare sample (1:1000), add 4 µL 2D-LC starter sample to 3996 µL
dilution solvent.
4 mL sample (1:1000) is prepared.
Parts required
p/n
Description
5190-6895
2D-LC starter sample, 1 x 2 mL
G2453-85060
Formic Acid-Reagent Grade 5 mL (5 cc)
858700-902
RRHD SB-C18, 2.1x100 mm, 1.8 µm, 1200 bar
In
1
D
857768-901
RRHD Bonus-RP, 2.1x50 mm, 1.8 µm, 1200 bar
In
2
D for Heart Cutting (LC-LC) and High Resolution (High Res)
959757-302
RRHD Eclipse Plus C18, 3.0x50 mm, 1.8 µm
In
2
D for Comprehensive 2D-LC (LCXLC)
Hardware required
Various hardware configurations are possible, see
Preparations
Take care that the following solvents for mobile phases are available:
•
1D:
•
A = water with 0.2 % Formic Acid-Reagent Grade 5 mL ( 5 cc) (G2453-85060)
•
B = methanol
•
2D:
•
A = water with 0.2 % Formic Acid-Reagent Grade 5 mL ( 5 cc) (G2453-85060)
•
B = acetonitrile
Содержание 1290 Infinity II 2D-LC Solution
Страница 122: ...5 2D LC Data Acquisition in MassHunter Workstation 11 Instrument Details 2D LC User Guide 122 3 Click Screen 4 Click OK...
Страница 263: ...2D LC User Guide 263 9 Data Analysis GC Image Basic Information 3 Import parameters...
Страница 264: ...9 Data Analysis GC Image Basic Information 2D LC User Guide 264 4 Fit view...
Страница 267: ...2D LC User Guide 267 9 Data Analysis GC Image Basic Information 11 View single 2D chromatograms 12 Select blobs...
