ELYRA 7
Center Screen Area / Image Containers - Display and Image Analysis
ZEISS
03/2019 V_02
000000-2262-999
373
By pressing
Clear all
all fiducials will be deselected. If
Clear all
is pressed or one of the fiducials is
deselected (
Use
box not checked) the
Select all
button becomes available. If
Select all
is pressed,
all fiducials will be selected and the button becomes inactive. The button also becomes inactive, if
all
Use
boxes are checked manually.
All identified fiducials (and those manually selected by
+
) are displayed as an overlay in the
HR
channel,
when the
Fiducials
box in the
Show
field is checked (Fig. 609). The overlay identifies the fiducial by
plotting its localizations by crosses and a circle around the localizations. The fiducial highlighted in the
Fiducials Table
has a circle with a thicker line.
Not all positions of a fiducial are displayed in the overlay, just every 1000th position is plotted, to
avoid crowding of the crosses in the image.
The color of the overlay can be selected by the
Color drop down
menu of the
Show
field. For more
color images
color drop down
menus for each channel will be available. The original identified localized
peaks corresponding to a fiducial will be deleted from the HR image (Fig. 610). The SWF channel will be
uninfluenced.
You can zoom into a fiducial by checking the
Zoom
box in the
Show
field. The software will zoom into
the fiducial that is currently highlighted in the Fiducials Table (Fig. 611).
Fig. 609
SMLM view - Fiducials displayed in the HR
channel
Fig. 610
SMLM view - Zoomed fiducial before (left) and
after (right) drift correction