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ELYRA 7
Left Tool Area and Hardware Control Tools
ZEISS
03/2019 V_02
000000-2262-999
127
5.2.17.2
Performing a Z-Stack Using
Center Mode
Press the
Center
mode button to open the
Center
definition
panel (Fig. 197). This mode allows
setting the Range of the Z-Stack in relation to the
current
Focus Position
which is indicated as
Offset
.
The values for number of
Slices
and
Interval
are
taken over from any settings done in
First/Last
mode. The
Offset
defines the
Center
position of
the stack in relation to the
Focus Position
.
The
Slices
input box displays the number of slices
of the stack. This number can be edited by typing
or using the arrow keys. Changing the value for
Slices
leaves the
Interval
untouched, but the total
Range
is adjusted.
The
Interval
input box displays the z-distance
(interval) between slices in
µm
. This number can be
edited by typing or using the arrow keys. Changing
the value for the
Interval
automatically adjusts the
number of
Slices
while adjusting the total
Range
.
The
Slices
number will remain unchanged.
Pressing the
(Opt)/(Leap)
button (only available in
the SIM Apotome and Lattice SIM
imaging modes
you can select between Optimal (Nyquist) and Leap (3 fold broader spacing). Optimal sets
Interval
to
match the Nyquist criteria (2 fold over sampling) and accordingly adjusts the
Slices
to keep the
Range
within the limits defined by
Set First
and
Set Last
. The optimal interval is displayed in the button in
µ
m
.
Leap sets the spacing 3 fold broader than Nyquist.
Within the
Keep
display field (only available in
Show all
mode) you have the option to switch between
Keep
Interval
and
Keep Slice
. If
Keep Interval
is checked, the number of slices is adjusted to a new
Range
defined pressing
Set First
or
Set Last
. Likewise, when
Keep Slice
is checked, the value for the
Interval
between the slices is adjusted to a new Range defined by pressing
Set First
or
Set Last
.
The
Position (
µ
m)
input box indicates the current position of the focus, no matter if the focus drive or a
Z-Piezo
stage insert was used for positioning. The arrow keys allow to change the focus in small steps. If
the
Use Piezo
check box is ticked (only available, if a
Z-Piezo
stage is attached to the system), the
Z-Piezo
stage insert will be used to perform all Z movements triggered from the software. If the focus
drive was used for
Set First
/
Set Last
, the system will transform this numbers for a matched
Z-Piezo
movement. In the
SIM
imaging mode, the
Use Piezo
check box is ticked by default and greyed out, so
there is no choice to deselect the box and use the microscope focus drive for
SIM
imaging. The rationale
behind this is that
SIM
acquisition needs the accuracy of a
Z-Piezo
stage insert for taking Z-Stacks.
Fig. 197
Z-Stack tool Center mode with
Z-Stack Navigation