ZEISS
Center Screen Area / Image Containers - Display and Image Analysis
ELYRA 7
360
000000-2262-999
03/2019 V_02
Thresholds tab (Fig. 577):
The threshold for the image analysis can be set
manually using the slider or editing text box next
to
Donor
,
Acceptor
,
FRET
or
All
, where
All
moves all sliders to the same value. Alternatively,
the threshold can be set by defining and enabling
a background region in the FRET tab. The values
for the thresholds are either displayed as grey value
levels (
Raw data
) or
Normalized
to the value 1.
Settings tab (Fig. 573 and Fig. 578):
This tab provides access to the definition of the
parameters used for the image analysis.
The
General
(Fig. 573) tab applies to both
methods, Acceptor Photobleaching and Sensitized
Emission.
If checked the listed options will be taken into
account for the analysis of FRET.
The
Sensitized Emission
(Fig. 578) tab sets
additional Truncation parameters for each method
to analyze the Sensitized Emission experiment such
as the choice for
No truncation
,
Remove
maximum
or the truncation of values above 1
(<=1), 2 (<=2) or 3 (<=3).
For the Fc analysis method, the option
Don’t
show negative values in the Fc image
can be
selected using the check box. With the
FRET-N
normalization
slider and text box a multiplication
factor for the calculated FRET-N values can be set.
Fig. 577
FRET View Options Control Block,
Thresholds tab
Fig. 578
FRET View Options Control Block,
Settings tab, Sensitized Emission