ZEISS
Left Tool Area and Hardware Control Tools
ELYRA 7
124
000000-2262-999
03/2019 V_02
5.2.17
Multidimensional Acquisition – Z-Stack
The
Z-Stack
tool tab is only available, if the
Z-Stack
checkbox is ticked in the
Multidimensional
acquisition
field. If the tool bar is expanded, the
Z-Stack
tool becomes available (Fig. 193, Fig. 194,
Fig. 195. When
Start Experiment
is pressed the system will perform a Z-Stack.
With the Z-Stack function a series of XY-images in
different focus positions can be acquired resulting
in a Z-Stack. In this way a 3 dimensional data set
from the specimen is obtained.
The way in which the Z-Stack is acquired can be
selected by pressing either the
First/Last
or
Center
mode buttons. The modes differ in the way
the first and last images of the stack are selected.
The
Center
mode is only available in the
Show all
mode.
In case a
Z-Piezo
stage insert focusing device is
attached to the system, the Piezo device can be
chosen for the acquisition of the stack.
Fig. 193
Z-Stack tool First/Last mode with
Z-Stack Navigation displayed for
the WF image mode
Fig. 194
Z-Stack tool First/Last mode with
Z-Stack Navigation displayed for
the Lattice SIM image mode
Fig. 195
Z-Stack tool First/Last mode with
Z-Stack Navigation displayed for
the Laser WF image mode