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ZEISS
Left Tool Area and Hardware Control Tools
ELYRA 7
204
000000-2262-999
03/2019 V_02
Once settings have been completed the
Preview
window displays beads that are not taken into account
as red circles and those that are used in white circles (if channel color is grey) or in their channel color (if
chanel color is other than grey) (Fig. 337). Press apply to compute the Experimental PSF. The created file
will have the extension "… .ExperimentalPSF. …".
Best results for an Experimental PSF for 3D data will be obtained, if beads are seeded sparsely, for
example 10 - 20 beads evenly distributed in a 512 x 512 image.
If
Apply
is pressed an average PSF from all the beads taken into account will be computed (Fig. 338). For
each channel a separate PSF will be displayed. The
Experimental PSF
can be saved (format .czi) and
used for the
Structured Illumination
- Structured Illumination
Processing tool in place of the
Theoretical PSF
. The size and pixel size of the
Experimental PSF
are recalculated and the PSF is
interpolated to match the processed image.
Fig. 337
Preview window for bead selection using 3D SMLM
Fig. 338
Experimental PSF in Ortho view