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ZEISS
Left Tool Area and Hardware Control Tools
ELYRA 7
208
000000-2262-999
03/2019 V_02
Noise Level
:
Noise Level
sets the threshold of the intensity of the sub-resolution beads over
background according to I – M > SD x NL (I = intensity of bead; M = man intensity of image frame; SD 0
standard deviation of image frame; NL = noise level). Lowest number means that everything (even noise)
is taken, highest number means only the brightest beads will be taken.
Beads quality
:
Beads quality
evaluates two characteristics, the intensity (zero moment) and the size
(second moment). These two moments are calculated for every localized bead. They basically will assess
the shape (aspect ratio of the beads). Then the beads are sorted according to these characteristics,
resulting in two sorted lists of beads. The beads that are closer to the medians in both orderings are
assumed to have higher quality. The Beads quality defines how far the beads characteristics (intensity and
size) may be away from the median value. The smaller the threshold, the closer beads should be to the
median. Beads quality equals to 0 is likely to result in a single bead right in the middle. Lower input
number means that only perfectly shaped round beads will be taken into account. Highest input numbers
will take into account all beads, even ellipsoid shaped ones.
All sub-resolution beads that will be taken will be circled by a white line. Those that do overlap with their
circles or that are considered unsuitable (beads beyond the quality threshold) are outlined in red and will
not be taken into account (Fig. 346).
Fig. 346
Selection of beads in Preview tab