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ELYRA 7
Left Tool Area and Hardware Control Tools
ZEISS
03/2019 V_02
000000-2262-999
191
5.3.15
Processing – Localization
Microscopy
The
Localization Microscopy
function allows
setting filters for peak finding and choosing a fit
model for the localization calculations.
Select the
Localization Microscopy
tab to select
the
Localization Microscopy
Processing tool
(Fig. 306). Activate the image you want to process
in the image container and press the
Select
button
to load the image. Then expand the
Method
Parameters
tool window to have access to the
Peak Finder
and
Localizer
Pressing the
Settings Default
button will reset all
filter and parameter settings to the default values.
Pressing the
Settings
Reuse
button will recall the
parameter settings of the actively selected image.
The
Channel
drop down menu allows you in a
multi-channel experiment to apply different settings
to different channels, which are all listed (Fig. 308).
If you select
All
, all channels will be fitted the same
way. If you select a channel, and change settings,
these setting will apply only to this channel. Note,
even if only one channel is selected, every channel
will be fitted according to the last settings.
The
Settings
drop down menu (Fig. 309) will allow
you to define how peak events will be fitted. You
have three options:
−
Discard overlapping molecules
(single
emitter): only single emitters will be taken
into account and fitted, whereas all multi-
emitter events will be discarded.
−
Ignore overlap
(ignore emitter): all single-
and multi-emitter events will be taken into
account and fitted as single emitter events.
Fig. 306
Processing – Localization Microscopy
Fig. 307
Processing – SMLM – SMLM
Fig. 308
Channel drop down menu of a dual
channel data set
Fig. 309
Settings drop dpwn menu