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ZEISS
Left Tool Area and Hardware Control Tools
ELYRA 7
126
000000-2262-999
03/2019 V_02
The rationale behind this is that
SIM
acquisition needs the accuracy of a
Z-Piezo
stage insert for taking Z-
Stacks.
The
Fast Z Line
checkbox is only available in
Line
Scan Mode
to be selected in the
Acquisition Mode
tool). It is not available with the
Z-Piezo
. If the check box is ticked and
Continuous
action button
pressed a fast XZ scan for overviews is performed using a continuous movement of the microscope focus.
The stack size is retained and the interval is adapted depending on the scan speed. The resulting center
of the Line-Z stack can deviate from the center set in frame imaging mode by some microns.
The
Z-Stack Navigation
field displays a graphical representation of the Z-Stack. The
white frames
represent the number and localization of the slices. The
blue frame
indicates
the current focus position,
also during image acquisition. By dragging the
blue frame
with the cursor, the current focus position
changes accordingly. The
ruler
immediate on the right indicates the dimensions (range) of the Z-Stack.
One can also change the focus position by using the slider tool right to the
ruler
. Click on the circle
symbol with the double arrow and move it up- or downwards to change the focus. This tool might also
be used for defining the first and last position for the Z-Stack.
The
F
,
C
and
L
buttons indicate the positions of the first slice, center slice and last slice of the stack,
respectively. If you press a button, a scan will be triggered at the respective focus position
It is not relevant that the first and last position in the software match the absolute first and last
position. The microscope will always move against gravity when acquiring the stack ignoring the
assignment first and last.
The precision of the Z-Stack position can be optimized for very critical applications by taking care
that the approach to the first and the last slice when marking the positions will be made from the
same direction as the Z-Stack is finally acquired. As the Z-Stack acquisition is always performed
against gravity it is recommended to move the objective (for inverted stand Axio Observer and
upright stand Axio Examiner) from a lower position to the first slice and then further up to the last
slice. During this process be sure to move the nosepiece in this one direction only and that you do
not refocus.
To obtain best image quality with Z-Stacks using the nosepiece and which are critical regarding the
focus impulse, a
Waitstate
can be activated. To this end go to the
Maintain
tab. In the
Option
field expand the
System Options
tab and select the
Hardware
tab. Tick the
Waitstate
check box.
Such a wait state is only beneficial at certain acquisition speeds and not needed by default.