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ELYRA 7
Center Screen Area / Image Containers - Display and Image Analysis
ZEISS
03/2019 V_02
000000-2262-999
351
Tools in the Unmixing View Options Control Block
−
Tools for display customization and drawing:
Drawing tools
work the same as in the Graphics View Option control
block
Show Table
button: The table of intensity values over Lambda is
displayed in the Image Display window.
Tables can always be saved or copied
to the clipboard by right-mouse clicking on the table
display!
−
Tools for unmixing:
Overview of spectral data selected, that is displayed and can be used for
unmixing. Spectra saved in the spectra database can be loaded (here:
Venus) by pressing the plus button in the upper right corner which
creates a new row. In this row the left black arrowhead opens the
spectra database. Manually defined ROIs (Region) and pixel selected by
Automatic Component Extraction (ACE) can be added.
Select ROI for background subtraction
Automatically balances the intensity of unmixed channels to equal
levels.
A statistical analysis of the signal-related (Poisson-) noise is performed
and weighs the respective contribution to the fitting of the combination
of reference spectra to the experimental data (see also section
).
An additional channel per unmixed component is displayed which
shows the relative standard deviation (RSD) in each unmixed component
channel (see also section
).
A separate channel in the unmixed image that displays the difference
between fit and original data in grey values for the channel of the
Lambda stack that shows the greatest deviation (see also section
The values of the intensity Lambda diagram are saved to the spectra
database in the ZEN\DyeDatabase directory. Make sure to have
displayed only data of single dyes in the intensity Lambda diagram when
executing this function. (The database interface is shown in Fig. 565).