ZEISS
Left Tool Area and Hardware Control Tools
ELYRA 7
130
000000-2262-999
03/2019 V_02
5.2.17.4
Correction
Expand the
Correction
field by pressing the
triangle (Fig. 199). The
Refractive Index
Correction input box
allows you to
consider the
different refractive indices between the immersion
medium of the objective (n') and the embedding
medium of the specimen (n), which can be set
between 0.5 and 3 by typing or using the arrow
keys. The number should correspond to the ratio
r=n/n’.
5.2.18
Multidimensional Acquisition – Tile
Scan
When the
Tile Scan
tool tab is expanded the
Tile
Scan
tool becomes available (Fig. 200).
This function permits a frame to be created as an
overview image of the specimen. The size of the
final tiled image depends on the settings for the
image format set in the
Acquisition Mode
tool
and the number of tiles.
The application of the
Tile Scan
function requires
an objective with a minimum magnification factor
of 2.5x.
The number of
Tiles
can be defined in the Tiles
Horizontal
and
Vertical
Input boxes. A maximum
of 100 tiles can be set. The size of the final Tile
Scan is displayed in
Pixels
and as a
Size
in
µ
m.
According to image format the number of
Pixels
changes. According to the objective used for
imaging the
Size
of the Tile Scan changes.
The Tile Scan grid is displayed in the
grid display
area. It shows the number and location of the
single tiles and also indicates any overlap.
The Tile scan is created around the absolute
starting position of the stage. In case the stage
does not move exactly horizontal to the scan field
a
Rotation
value can be set in the
Rotation
input
box to correct for this (available only in
Show all
mode). This can either be calibrated beforehand
using a grid slide or any other specimen which
displays clearly visible structures in horizontal
direction.
Fig. 199
Correction field – appearance for
the WF, SIM and Laser WF imaging
modes
Fig. 200
Tile Scan tool – Centered grid active
Fig. 201
Tile Scan tool – Overlap defined