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ELYRA 7
Left Tool Area and Hardware Control Tools
ZEISS
03/2019 V_02
000000-2262-999
177
Ratiometric Dyes - Calibration
•
Subtract background/autofluorescence images from raw images to obtain
R
korr
[=F
1
-F
1Background
)/(F
2
-F
2Background
)]
when calibration reference is not obtained with the experimental sample (in situ).
•
Calculate ratio R.
•
Perform equation- or titration calibration (compare R with a calibration curve
→
titration calibration or
put R values in calibration formula).
Fig. 281
Ion concentration panel