Thermo Scientific Dionex, Operator'S Manual

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Dionex Integral Systems Operator’s Manual
90
Doc. 065250-06 03/2020
6. Using a syringe (P/N 016640) and a luer adapter (P/N 24305), fill the
standard loop with deionized water. Do not introduce any air into the
loop.
7. Reinstall the plug on the end of the coupler from which the water
exited.
8. Remove the syringe and luer adapter from the other end of the loop
and install the plug in it.
9. Examine the outside of the loop for water droplets. Carefully dry any
water and then weigh the loop to the nearest 0.0001gram, if possible.
10. Subtract the weight of the empty loop ( Step 3 ) from the weight of the
filled loop ( Step 9 ); the difference is the weight of the water in the
standard loop.
11. Repeat Step 9 and Step 10 until four to five consecutive weighings

0.009 are achieved.
12. Multiply the weight of the water by 1000 to obtain the standard loop
volume in microliters (

L). The table below lists examples of dilution
factors and final concentrations of the diluted standard.
Standard
Loop Size
(

L)
Dilution
Volume
(mL) Dilution
Factor Calibration
Standard
Concentration
(mg/L) Diluted
Standard
Concentration
(

g/L)
10 50 5000 10 2
20 50 2500 10 4
25 50 2000 10 5
50 50 1000 10 10
100 50 500 10 20
150 50 33 10 30
200 50 250 10 40
250 50 250 10 40
250 25 100 10 100