Thermo Scientific Dionex, Operator'S Manual

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7 • Startup, Operation, and Shutdown
Doc. 065250-06 03/2020
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7.2.2 Set Up Chromeleon 7 PA and Configure the System
•
For installation instructions for Chromeleon 7 PA, refer to
Chromeleon 7.3 Installation Guide (Document No. 7329.0003).
•
For instructions on how to configure the system, refer to the
Chromeleon 7 PA Help.
7.2.3 Flush the Flow Path
1. Open the gas inlet valves.
2. Adjust the pressure of the regulator on the SP panel to 170 kPa
(25 psi).
3. Set the LE controls to
VENT
. Adjust the pressure of the regulator on
the LE to 34 kPa (5 psi).
4. Prepare mobile phases, standards, and reagents (if used). Fill
containers and pressurize to the appropriate pressures. Refer to the
NOWPak documentation provided with the NOWPak Bag-in-a-
Bottle containers (P/N AAA-068551) for filling instructions. Open
water supplies to each system.
5. Manually actuate the SP valves to flush the lines with water, diluent,
sample, and standards.
6. Fill the dilution vessel with water or diluent and then drain the liquid.
Depending on the application, it may be necessary to repeat this
process several times—or soak the vessel overnight—in order to
remove trace contaminants.
7. Before installing consumable components (see Section 7.2.4 ), set the
analytical pump flow rate to 0.25 mL/min (for a microbore pump) or
1.0 mL/min (for a standard bore pump). Prime and start the pump to
flush the chromatography flow path. If a suppressor is being used
with external water for regenerant, flush these lines, also.
8. While pumping through the chromatography flow path (without
consumables), confirm that the total backpressure remains below
690 kPa (100 psi).