Qiagen QIAgility, User Manual

Page: 243 / 334

Instrument Setup and Calibration
QIAgility User Manual 11/2011 7-9
7. Click on the “OK” button to continue.
8. If “Require confirmation from user?” was selected, the
following message will appear.
9. Click on the “Yes” button to accept the calibration, if the
averages for a plate are within 10 units of each other. If
the averages are not within 10 units of each other, click
on the “No” button to reject the current calibration and
then repeat steps 5–8.
Note : Before repeating the calibration, ensure that all
tubes are sitting correctly and that the tips are not bent.
Note : If there is significant variation across the 4
samples, the following message box will appear. Click
on the “OK” or the “Cancel” button.
10. If more than one plate was selected in the “Plates to
Calibrate” list, the instrument will calibrate each plate in
turn. Repeat steps 6–9 for each plate.
11. When all calibrations are complete, click on the “Close”
button.
Manual plate height calibration
1. Complete steps 1–4 of “Automatic plate height
calibration”.
2. Click on the “Manual Setup (highlighted item)” button.
3. A dialog box will appear, click on the “Yes” button to
continue.