Qiagen QIAgility, User Manual

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Glossary
QIAgility User Manual 11/2011 10-1
10 Glossary
Term Description
Diluent Diluent is used to dilute the master mix, dilute samples,
and create standard dilution series. It is assumed to be
PCR-grade water. A diluent can only be assigned to a
single well segment. Only 1 diluent tube can be assigned
in a run file.
Lid The instrument lid isolates the worktable, maintaining
sample integrity, and acting as a safety mechanism.
Master mix A master mix is a pooled group of reagents that can be
added to a reaction plate. Master mix is always added to a
reaction first because it normally constitutes the largest
volume of the reaction.
Master Mix
Block
The Master Mix Block is intended to hold diluent and
master mix on the instrument worktable.
Menu bar The menu bar contains 5 menus — “File”, “Control”,
“Wizards”, “Options”, and “Help”. All software functions
can be controlled from these menus.
Mode
indicator
Indicates whether the instrument is operating in “Normal
Mode” or “Virtual Mode”.
Normal mode For “Normal Mode” operation the instrument must be
switched on and connected to the computer. In “Normal
Mode” operation, run files can be executed.
Power switch A button located at the back of the QIAgility. It allows the
user to switch the QIAgility on and off.
Q Protocol Q Protocols are run files which have been developed by
QIAGEN to directly facilitate specific PCR applications. Q
Protocols are installed in the “QProtocols” subfolder