Qiagen QIAgility, User Manual

Page: 205 / 334

QIAgility Software
QIAgility User Manual 11/2011 5-143
Master mix with no sample
1. Select “No samples (empty wells)” from the “Samples”
panel.
2. Select the master mix.
3. Enter the number of replicates in the “Target wells”
panel. The number of replicates will equal the number of
wells in the reaction plate.
4. Click on the “OK” button.
Master mix with standard
1. Select the dilution series from the “Samples” panel.
2. Select the master mix.
3. Enter the number of replicates in the “Target wells”
panel.
4. Click on the “OK” button.
Master mix with sample bank
1. Select the sample bank from the “Samples” panel.
2. Select the master mix.
3. Enter the number of replicates in the “Target wells”
panel.
4. Click on the “OK” button.
Sample bank with no master mix
1. Select the sample bank from the “Samples” panel.
2. Set the sample volume in the “Volume” panel.
3. Enter the number of replicates in the “Target wells”
panel.
4. Click on the “OK” button.
Sample bank with reagents not used in master mix
1. Select the sample bank from the “Samples” panel.
2. Select the reagents from the “Unmixed reagents” panel.