Qiagen QIAgility, User Manual

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QIAgility Software
5-106 QIAgility User Manual 11/2011
Change tips
after each
dilution?
Instructs the instrument to change tips after
each dilution. When selected, a new tip is
used for aliquoting each diluent volume
and each dilution sample. When not
selected, a single tip is used to aliquot
diluent, and a new tip is used to aliquot
the dilution samples.
No. of times to
mix
Defines the number of times to mix.
Ratio Defines the sample:diluent ratio for a given
dilution.
Add Adds a given dilution to the “Dilutions” list.
Remove Removes a selected dilution from the
“Dilutions” list.
7. Select the diluent and the sample bank to be used in the
serial dilution from the drop-down menus.
8. Click on the button to select the target reaction plate.
The “Plate/well Selector” window will appear.
9. Left-click on a reaction plate to select it. The “Serial
Dilution” dialog box will re-appear.
Note : If there is only one “Reaction” plate, this plate will
be selected by default. To return to the “Serial Dilution”
dialog box, left-click on the selected plate.
10. Define the options as required in the “Special settings for
Dilutions” panel.
11. Define a series of dilution ratios. To do this, enter a ratio
and then click on the “Add” button. Repeat this for every
dilution ratio of the series.
Note : To remove a dilution, left-click on a copy in the
“Dilutions” list, and then click on the “Remove” button.
12. Click on the “OK” button to return to the main software
window. The dilutions will be added to the “Reaction
List”.