Qiagen QIAgility, User Manual

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QIAgility Software
5-104 QIAgility User Manual 11/2011
8. Click on the button to select the target reaction plate.
The “Plate/well Selector” window will appear.
9. Left-click on a reaction plate to select it. The “Sample
Dilution” dialog box will reappear.
Note : If there is only one “Reaction” plate, this plate will
be selected by default. Left-click on the selected plate to
return to the “Sample Dilution” dialog box.
10. Define the “First well”.
11. Define the “Sample volume” and the “Diluent volume”,
and then click on the “Add” button.
12. If required, repeat steps 8–12 to configure more
dilutions.
Note : To remove a copy, left-click on a copy in the
“Reaction plates” list, and then click on the “Remove”
button.
13. Click on the “OK” button to return to the main software
window. The “Reaction List” will be updated with the
sample dilution step.
Add serial dilution step
1. Create a sample bank that contains 1 well. See
Section 5.8.1 for details about how to do this.
2. Left-click on a “Reaction” plate.
3. The “Reaction” right-hand pane will appear.
4. Click on the “Special” button.
5. Select “Add serial dilution step”.
6. The “Serial Dilution” dialog box will appear.