Qiagen QIAgility, User Manual

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Appendix F
F-6 QIAgility User Manual 11/2011
Use optimal height calibration where possible
As the mechanism used for automatic height calibration
(Section 7.1.3) must account for several sources of
measurement error, the software implements a
compensation factor to ensure that tips do not contact the
tube/well base during normal operation. As a consequence,
the dead volumes for some tubes can be larger than is
desirable.
This function activates an alternate height calibration
mechanism which tries to optimize the calibrated tube/well
height more closely to the true internal base of the vessel.
Details are described below. While this may reduce the
wasted dead volume, it also increases the risk that liquid may
not be found during aspirate operations. Consequently, this
function is offered only for selected vessels.
By default, this function is not activated.
Optimal plate height calibration
This function is activated by the ‘use optimal height
calibration where possible’ advanced setting.
Before starting the procedure:

Ensure that 50 µl tips are available, that the Tip Rack
Holder is calibrated, and that the correct tips are set as
available.
1. Select “Options/Robot Setup/Calibrate plate height”.
2. The “Configure Plate Heights” dialog box will appear.