Qiagen QIAgility, User Manual

Page: 223 / 334

Operating Procedures
QIAgility User Manual 11/2011 6-11
5. Load the correct plate height and plate position
calibrations for each plate and Tip Rack Holder. See
Sections 5.5.2, 7.1.2, and 7.1.3 for more details about
how to do this.
6. Place the correct tubes in each plate on the worktable.
7. Add the correct volumes of reagent, diluent, standard,
samples, and, if used, premixed master mix into the
correct tubes on the worktable.
Note : To view the required volume of liquid, place the
cursor over the source well. A hover box will appear
which displays the required volume of liquid that the
software has calculated. This volume includes the extra
volume which allows for liquid recovery limitations and
losses due to evaporation and tip contact during
pipetting. Alternatively, view the pre-run report.
8. Close the instrument lid.
6.4.5 Starting a run
1. Click on the icon on the toolbar, or select
“Control/Start”.
2. The “Save as” dialog box will appear. To save the run
file, enter a name for the run file and click on the “Save”
button. If the run file has already been saved, click on the
“Cancel” button to continue.
3. The pre-run “Checklist” dialog box will appear.